Phospho-MEF2C-S396 Rabbit pAb - Q06413

Core Product Specifications and Parameters

Biological Background: MEF2C Function and Localization

• MEF2C is a transcription activator that binds to the MEF2 element in regulatory regions of many muscle-specific genes, controlling cardiac morphogenesis and myogenesis.
• It is also involved in vascular development and enhances transcriptional activation mediated by SOX18.
• MEF2C plays an essential role in hippocampal-dependent learning and memory by regulating excitatory synapse number and synaptic transmission.
• Crucial for normal neuronal development, distribution, and electrical activity in the neocortex, and may be involved in neurogenesis and cortical architecture (By similarity).
• Necessary for proper development of megakaryocytes and platelets, as well as bone marrow B-lymphopoiesis.
• Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens, and normal induction of germinal center B-cells.
• Isoforms lacking the repressor domain are more active than isoform 1 (alternative splicing).
• Subcellular localization includes nucleus, cytoplasm, and sarcoplasm; tissue expression in brain and skeletal muscle.

Experimental Guidance and Technical Tips

• For Western blot, treat cell lysates with lambda phosphatase to confirm phosphorylation-specific signal; expect a band around 51 kDa.
• Validate antibody specificity in relevant human cell lines or tissues expressing MEF2C, such as neuronal or skeletal muscle cells.
• For ELISA applications, consider using phospho-peptide and non-phospho-peptide as coating antigens to confirm phospho-specificity.
• Since MEF2C has multiple isoforms, verify detection of the targeted isoform using appropriate controls or recombinant proteins.
• Optimize antibody concentration for each application; recommended starting dilutions can be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For studying MEF2C phosphorylation and its roles in neurodevelopment, myogenesis, and immune regulation, we offer validated antibodies, bulk ancillary reagents, and rare-target sourcing to streamline your assay development. Contact us to support your procurement needs with reliable, high-quality materials.