Phospho-MDM2-S260 Rabbit pAb for WB, ELISA - Q00987

Core Product Specifications and Parameters

Biological Background: MDM2 Function and Localization

• MDM2 is an E3 ubiquitin-protein ligase that primarily targets p53/TP53 for ubiquitination and proteasomal degradation, inhibiting p53-mediated cell cycle arrest and apoptosis. Related references: PMID:29681526
• It also ubiquitinates other substrates including IGF1R, SNAI1, DYRK2, and DCX, regulating processes such as receptor degradation, neuronal development, and cell migration. Related references: PMID:12821780, PMID:15053880, PMID:15195100
• MDM2 negatively regulates NDUFS1, leading to decreased mitochondrial respiration, increased oxidative stress, and commitment to the mitochondrial pathway of apoptosis. Related references: PMID:30879903
• Subcellularly, MDM2 is found in the nucleus, nucleoplasm, cytoplasm, and nucleolus, shuttling between compartments to exert its diverse functions.
• MDM2 is a phosphoprotein with a RING-type zinc finger domain; phosphorylation at multiple sites, including S260, may modulate its activity, stability, and interactions with p53 and other partners.
• Tissue-specifically, MDM2 is ubiquitously expressed, but certain splice isoforms (Mdm2-A through -G) are observed in a range of cancers while absent in normal tissues.

Experimental Guidance and Technical Tips

• Use phosphatase inhibitors during sample preparation to preserve the phosphorylation at S260.
• For WB, consider blocking with non-phosphorylated peptide or treating samples with phosphatase to confirm phospho-specificity.
• Validate the antibody in human cell lines or tissues with known MDM2 expression and phosphorylation status (e.g., cancer cells with wild-type p53 under genotoxic stress).
• For ELISA, ensure the capture and detection system maintains the phosphorylated epitope and avoid prolonged incubations that may lead to dephosphorylation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, covering every stage from concept to clinic. This Phospho-MDM2-S260 Rabbit pAb supports research into the p53 pathway and cancer biology. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare targets to meet your diagnostic development needs.