Phospho-MCM2-S139 Rabbit Polyclonal Antibody for WB, ELISA - P49736

Core Product Specifications and Parameters

Biological Background: MCM2 Function and Localization

• MCM2 is a core component of the MCM2-7 complex, which functions as the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation. As part of the CDC45-MCM-GINS (CMG) helicase, it unwinds template DNA around which the replisome is built. Related references: PMID:32453425, PMID:34694004, PMID:34700328
• MCM2 is required for entry into S phase and for cell division. Related references: PMID:8175912
• The protein localizes to the nucleus and chromosomes, consistent with its role in DNA replication.
• MCM2 is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may regulate its activity.
• It contains ATP-binding and zinc-finger domains, functioning as an ATP-dependent helicase.
• MCM2 plays a role in the development of terminally differentiated hair cells in the cochlea and can induce apoptosis. Related references: PMID:26196677
• Mutations in MCM2 are associated with non-syndromic deafness (DFNA70).

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using a primary antibody dilution optimized for your sample and detection system; MCM2 runs at approximately 102 kDa.
• To confirm phospho-specificity, treat samples with a phosphatase and verify loss of the signal.
• Use appropriate positive and negative controls, such as untreated versus phosphatase-treated cell lysates.
• For ELISA, titrate the antibody to determine the optimal coating concentration and detection conditions.
• Validate cross-reactivity with your species of interest if not human; reactivity with other species has not been determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents, supporting your DNA replication and cell cycle research needs. With expertise in sourcing rare and phospho-specific antibodies like this anti-Phospho-MCM2-S139 polyclonal antibody, we streamline your assay development from discovery to clinical application. Let CamelBio be your trusted partner in bridging the gap between concept and clinic.