Phospho-MCL1-T163 Rabbit pAb for WB, ELISA - Q07820

Core Product Specifications and Parameters

Biological Background: MCL1 Function and Localization

• MCL1 (Induced myeloid leukemia cell differentiation protein Mcl-1, also known as Bcl2-L-3) is an anti-apoptotic member of the Bcl-2 family, critical for cell survival.
• Alternative splicing generates pro-survival (isoform 1) and pro-apoptotic (isoform 2) variants, balancing apoptosis regulation.
• Core function: Inhibits apoptosis by binding and neutralizing pro-apoptotic Bcl-2 family members, maintaining cell viability without promoting proliferation.
• Subcellular localization: Found in cytoplasm, mitochondria, nucleus, and membrane; translocates to mitochondria to exert its anti-apoptotic effects.
• Post-translational modifications: Phosphorylation at multiple sites (including T163) regulates protein stability and anti-apoptotic activity; also subject to ubiquitination and isopeptide bond formation.
• Keywords: Phosphoprotein, alternative splicing, apoptosis, differentiation, mitochondrial membrane, transmembrane helix, developmental protein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to residues around T163 of human MCL1. Confirm specificity by comparing signals from phosphatase-treated versus untreated samples in WB.
• For WB, the predicted molecular weight is 37 kDa. Consider using a phospho-specific protocol with BSA-based blocking to reduce background.
• Validate antibody reactivity in both human and mouse cell lines known to express MCL1; cross-reactivity with other species has not been tested.
• In ELISA, use a phosphorylated peptide as coating antigen and a non-phosphorylated peptide as negative control to ensure phospho-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, bridging diagnostic manufacturers with high-quality reagents like this phospho-MCL1-T163 antibody, which supports apoptosis and leukemia research. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials—backed by comprehensive technical and consulting services to accelerate your development from concept to clinic.