Phospho-MAX-S11 Rabbit Polyclonal Antibody for WB, ELISA - P61244

Core Product Specifications and Parameters

Biological Background: MAX Function and Localization

• Protein max (Myc-associated factor X) is a class D basic helix-loop-helix protein (bHLHd4) that functions as a transcription regulator.
• Forms sequence-specific DNA-binding heterodimers with MYC or MAD, recognizing the E-box motif 5'-CAC[GA]TG-3'. The MYC:MAX complex activates transcription, while MAD:MAX represses it.
• Can repress transcription by recruiting a chromatin remodeling complex with H3 'Lys-9' histone methyltransferase activity.
• Represses MYC transcriptional activity from E-box elements.
• Localizes to the nucleus and is also found in cell projections, specifically dendrites.
• Tissue expression is highest in brain, heart, and lung, with lower levels in liver, kidney, and skeletal muscle.
• Key post-translational modifications include phosphorylation (at Ser-11 among other sites), acetylation, and alternative splicing. Classified as a phosphoprotein and DNA-binding factor.
• Implicated in disease variants and serves as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser11; therefore, this antibody specifically recognizes the phosphorylated form of MAX at S11. Verify specificity using non-phosphorylated peptide controls in ELISA or peptide competition assays.
• For Western blot (WB), the predicted molecular weight is approximately 18 kDa. Use appropriate protein lysis buffers containing phosphatase inhibitors to maintain phosphorylation status.
• As a rabbit polyclonal antibody, it may exhibit lot-to-lot variability; validate each new lot in the intended application.
• For ELISA, consider coating with the phosphopeptide antigen and use a blocking buffer to minimize nonspecific binding. A phosphopeptide-BSA conjugate may improve detection.
• Cross-reactivity is reported with human MAX; evaluate reactivity in relevant human cell lines or tissue samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop sourcing partner for IVD raw materials, supporting transcription factor research with validated antibodies like this phospho-MAX-S11 pAb. Our portfolio includes optimized monoclonal/polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents to accelerate your assay development. For rare targets or specialized phospho-specific reagents, CamelBio leverages extensive networks to bridge procurement from discovery to clinical applications.