Phospho-MARCKS-S162 Rabbit Polyclonal Antibody for WB, ELISA - P29966

Core Product Specifications and Parameters

Biological Background: MARCKS Function and Localization

• MARCKS (Myristoylated alanine-rich C-kinase substrate), also known as MACS, 80K-L, or PKCSL, is a widely expressed intracellular protein that serves as a major substrate for protein kinase C (PKC).
• It plays a central role in modulating the actin cytoskeleton, thereby regulating processes such as chemotaxis, motility, cell adhesion, phagocytosis, and exocytosis (PubMed:23704996, PubMed:36009319).

Related references: PMID:23704996 PMID:36009319

• In quiescent cells, MARCKS sequesters phosphatidylinositol 4,5-bisphosphate (PIP2) at plasma membrane lipid rafts; phosphorylation by PKC reverses this sequestration, promoting exocytosis (PubMed:23704996).

Related references: PMID:23704996

• During inflammation, MARCKS facilitates the migration and adhesion of inflammatory cells and enhances the secretion of cytokines such as TNF, particularly in macrophages (PubMed:37949888).

Related references: PMID:37949888

• The protein is involved in bacteria-induced intracellular reactive oxygen species (ROS) formation in monocytic cells.
• MARCKS localizes to the cell membrane, cytoplasm, and cytoskeleton, reflecting its dynamic roles in membrane-cytoskeletal interactions.
• Detected in spermatozoa, suggesting functions in male fertility and sperm motility.
• Post-translational modifications include N-terminal myristoylation, acetylation, and extensive phosphorylation; it also contains actin- and calmodulin-binding domains.

Experimental Guidance and Technical Tips

• Since this antibody was raised against a phospho-specific peptide surrounding Ser162, it is recommended to confirm phospho-specificity using phosphatase-treated samples or by comparing signals with a pan-MARCKS antibody in WB.
• For WB, consider using whole-cell lysates from stimulated vs. unstimulated cells (e.g., PMA treatment to activate PKC) to validate detection of the phosphorylated form.
• In ELISA, the antibody may be used to detect phospho-MARCKS-S162 in peptide or protein coating formats; appropriate blocking agents and controls are advised.
• Optimal working concentrations should be determined empirically by the end user for each specific assay and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and biotech teams with streamlined access to a comprehensive portfolio of IVD raw materials—including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For projects involving PKC signaling, cytoskeletal remodeling, or inflammation, we can supply phospho-specific and total MARCKS antibodies along with technical consultation to support your assay development. Our integrated platform bridges the gap from concept to clinic, ensuring consistent quality and supply chain reliability for your research and diagnostic needs.