Phospho-MAP3K5-T845 Rabbit Polyclonal Antibody for WB, ELISA - O35099

Core Product Specifications and Parameters

Biological Background: MAP3K5 Function and Localization

• MAP3K5, also known as ASK-1 or MEKK5, is a serine/threonine kinase that acts as a central component of the MAP kinase signal transduction pathway.
• It mediates cellular responses to environmental changes, regulating cell fate decisions including differentiation, survival, and apoptosis via mitochondria-dependent caspase activation.
• MAP3K5 is crucial for innate immunity, transducing signals from oxidative stress and inflammatory mediators like TNF and LPS.
• Upon activation, it phosphorylates MAP kinase kinases (MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6, MAP2K7/MKK7), which then activate p38 MAPK and JNK, leading to transcription factor AP-1 regulation.
• The protein localizes to the cytoplasm and endoplasmic reticulum.
• It is widely expressed in adult mouse tissues such as heart, brain, lung, liver, and kidney.
• Key features include ATP-binding, coiled-coil domains, and regulation by phosphorylation and ubiquitination; it plays roles in stress response, apoptosis, and immune signaling.

Experimental Guidance and Technical Tips

• This antibody is designed to detect phosphorylation at T845 of mouse MAP3K5; it cross-reacts with human species, as indicated.
• For Western blotting, consider using phosphatase inhibitors during sample preparation to preserve phosphorylation signals and validate bands using appropriate controls.
• ELISA may require optimization of coating antigen and antibody titers; use a phosphorylated peptide standard for calibration.
• We recommend empirically determining the optimal working dilution in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio is your one-stop partner for IVD raw material sourcing, offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents from concept to clinic.
• This phospho-MAP3K5-T845 antibody is ideal for research and diagnostic development in stress signaling, apoptosis, and innate immunity, with cross-species utility.
• We also provide custom antibody services and bulk reagent supply to support your IVD manufacturing and assay development needs.