Phospho-MAP3K5-S83 Rabbit pAb for WB, ELISA - Q99683

Core Product Specifications and Parameters

Biological Background: MAP3K5 Function and Localization

• MAP3K5 (Mitogen-activated protein kinase kinase kinase 5), also known as ASK-1, MEKK5, or MAPKKK5, is a serine/threonine kinase.
• It functions as an essential component of the MAP kinase signal transduction pathway, mediating cellular responses to environmental changes, including stress, cytokines, and LPS.
• MAP3K5 plays a crucial role in apoptosis signal transduction through mitochondria-dependent caspase activation and is required for innate immune responses against pathogens.
• Upon activation, MAP3K5 phosphorylates and activates MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6, and MAP2K7/MKK7, which then activate p38 MAPKs and JNKs, ultimately regulating AP-1 transcription factors.
• Subcellular localization includes the cytoplasm and endoplasmic reticulum.
• It is abundantly expressed in heart and pancreas, with alternative splicing reported.
• Key post-translational modifications and features include phosphorylation, methylation, ubiquitination, and ATP binding, highlighting its regulatory role in stress responses, apoptosis, and host-virus interactions.

Experimental Guidance and Technical Tips

• This antibody is generated against a synthetic phospho-peptide around S83 of human MAP3K5; for Western blot (WB), consider using phospho-specific detection and validate band size around 155kDa in relevant human cell lysates stimulated to induce phosphorylation.
• For ELISA applications, the phospho-peptide immunogen can serve as a positive control to assess binding specificity; include non-phosphorylated peptide controls to confirm phospho-specificity.
• Given MAP3K5's role in stress and inflammatory signaling, consider activating pathways (e.g., oxidative stress, TNF-α) in your experimental system to enrich phosphorylated targets prior to analysis.
• As cross-reactivity is reported for human, validate reactivity in your specific human tissue or cell type of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials, including validated antibodies for apoptosis and MAPK signaling research. With this Phospho-MAP3K5-S83 Rabbit pAb, researchers can source a critical tool for studying stress response pathways, backed by CamelBio’s supply capabilities in monoclonal/polyclonal antibodies, bulk reagents, and rare target sourcing. Our one-stop platform supports your IVD development from concept to clinic with reliable ancillary materials and technical consulting.