Phospho-MAP3K5 (ASK1/MEKK5) S967 Rabbit Polyclonal Antibody for WB and ELISA - Q99683

Core Product Specifications and Parameters

Biological Background: MAP3K5 (ASK1/MEKK5) Function and Localization

• Also known as Apoptosis signal-regulating kinase 1 (ASK-1) and MAPK/ERK kinase kinase 5 (MEKK5), MAP3K5 is a serine/threonine kinase essential for MAP kinase signal transduction.
• Functions as a key mediator of cellular stress responses, including oxidative stress, and receptor-mediated inflammatory signals such as TNF and LPS.
• Plays a crucial role in apoptosis by activating the JNK and p38 MAPK pathways through phosphorylation of MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6, and MAP2K7/MKK7.
• Required for innate immune responses, contributing to host defense against a broad range of pathogens.
• Localizes predominantly in the cytoplasm and endoplasmic reticulum.
• Abundantly expressed in heart and pancreas tissues.
• Undergoes phosphorylation at multiple sites, including S967, and is regulated by post-translational modifications such as methylation and ubiquitination (as indicated by keywords).

Experimental Guidance and Technical Tips

• The immunogen is a phospho-specific peptide corresponding to residues surrounding S967 of human MAP3K5. For Western blotting, consider treating cell lysates with phosphatase to confirm signal specificity, and optionally block with non-phosphorylated peptide.
• Due to the high molecular weight (155 kDa), ensure adequate gel separation and transfer conditions for clear visualization.
• For ELISA, use phospho-peptide versus non-phospho peptide as coating antigens to verify phospho-specificity.
• The antibody is listed for WB and ELISA; validation in other applications may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality antibodies and ancillary reagents. This phospho-specific anti-MAP3K5 (ASK1) antibody supports apoptosis, stress signaling, and innate immunity research. Beyond this product, we offer validated antibody pairs, bulk recombinant proteins, and customized procurement for rare targets to streamline your development pipeline.