Phospho-MAP2K6-S207/MAP2K3-S189 Rabbit pAb for WB - P46734 / P52564

Core Product Specifications and Parameters

Biological Background: Dual specificity mitogen-activated protein kinase kinase 3 (MAP2K3) Function and Localization

• Encoded by the MAP2K3 gene (also known as MEK3, MKK3, PRKMK3, or SKK2); functions as a dual specificity mitogen-activated protein kinase kinase that catalyzes the concomitant phosphorylation of threonine and tyrosine residues in p38 MAP kinases.
• Activated by pro-inflammatory cytokines and environmental stress; plays a central role in the p38 MAP kinase signaling cascade, mediating cellular responses such as inflammation, apoptosis, and differentiation.
• Participates in the ADRA1B adrenergic receptor signaling pathway, linking G protein-coupled receptor activation to downstream MAPK14 effects.
• Alternative splicing generates multiple isoforms, which may contribute to differential tissue expression or fine-tuning of kinase activity.
• Post-translationally regulated by acetylation and phosphorylation, with phosphorylation at Ser189 (and the analogous Ser207 in MAP2K6) being critical for kinase activation.
• Widely expressed in human tissues, with particularly high abundance in skeletal muscle, consistent with its role in stress-responsive signaling.
• Classified as a phosphoprotein and a disease variant; mutations in MAP2K3 have been associated with pathological conditions.

Experimental Guidance and Technical Tips

• Consider using this antibody for Western blot and ELISA; for all applications, validate specificity in your experimental system under appropriate controls.
• To detect phosphorylation-dependent signals, treat cells with known activators of the p38 pathway (e.g., anisomycin, TNF-α, or UV irradiation) and include phosphatase inhibitor cocktails during sample preparation.
• When performing Western blot, probe with a total MAP2K3/MAP2K6 antibody in parallel to confirm equal loading and assess phosphorylation stoichiometry.
• Due to sequence homology, the antibody may also recognize phosphorylated forms of related MAP2Ks; verify cross-reactivity by performing peptide competition or siRNA knockdown assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For researchers and diagnostic developers focusing on the MAPK signaling pathway, we provide reliable access to phosphorylation-specific detection tools and can assist with custom sourcing of rare-target raw materials. Contact us to learn how we can support your project from concept to clinic.