Phospho-MAP2K4-S257 Rabbit pAb for Western Blot, ELISA - P45985

Core Product Specifications and Parameters

Biological Background: MAP2K4 Function and Localization

• Dual specificity protein kinase acting as an essential component of the MAP kinase signal transduction pathway.
• Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway; directly phosphorylates and activates MAPK8/JNK1, MAPK9/JNK2, and MAPK10/JNK3.
• Along with MAP2K7/MKK7, is one of the only known kinases to directly activate JNKs; MAP2K4 preferentially phosphorylates the Tyr residue within the Thr-Pro-Tyr motif.
• Additionally activates p38 MAPKs (MAPK11, MAPK12, MAPK13, MAPK14), unlike MAP2K7 which exclusively targets JNKs.
• Required for maintaining peripheral lymphoid homeostasis.
• Participates in mitochondrial death signaling, promoting cytochrome c release and apoptosis.
• Subcellular localization includes both cytoplasm and nucleus.
• Abundantly expressed in skeletal muscle, with wide distribution across other tissues.

Experimental Guidance and Technical Tips

• Confirm phospho-specificity by treating samples with lambda phosphatase or by co-incubation with non-phosphorylated blocking peptide.
• Include appropriate positive and negative control lysates (e.g., stimulated versus unstimulated cells) to validate phospho-signal in WB.
• For mouse or rat samples, verify cross-reactivity and sequence alignment at the phosphorylation site before quantitative assays.
• Determine optimal antibody dilution for each application experimentally; typical starting dilutions can be found in the antibody datasheet.
• In ELISA, ensure consistent plate coating and detection reagents to minimize variability in phospho-quantification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with a streamlined sourcing hub for IVD raw materials, including validated phospho-specific antibodies such as this MAP2K4 pAb, supporting stress kinase signaling studies and assay development. Our portfolio encompasses optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and access to rare-target raw materials, ensuring end-to-end support from discovery to clinical application. Let CamelBio bridge your procurement needs with reliable supply and technical expertise.