Phospho-MAP2K3-S189 + MAP2K6-S207 Rabbit pAb for WB, ELISA - P46734 / P52564

Core Product Specifications and Parameters

Biological Background: MAP2K3 Function and Localization

• MAP2K3 (MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2) is a dual specificity protein kinase that phosphorylates both threonine and tyrosine residues, primarily targeting p38 MAP kinase.
• It is activated by cytokines and environmental stress, functioning as a key component of the stress-activated protein kinase signaling cascade, linking adrenergic receptor ADRA1B stimulation to MAPK14 activation.
• The protein contains conserved ATP-binding and nucleotide-binding domains, characteristic of serine/threonine and tyrosine protein kinases, enabling its catalytic transferase activity.
• MAP2K3 is subject to post-translational acetylation and alternative splicing, and has been identified in proteomic analyses as a phosphoprotein.
• It is widely expressed across human tissues, with particularly high abundance in skeletal muscle, reflecting its role in stress-responsive signal transduction.
• Genetic variations in MAP2K3 have been cataloged as disease variants, suggesting its involvement in pathological conditions.

Experimental Guidance and Technical Tips

• This antibody was generated against a synthetic phosphopeptide corresponding to phosphorylation site S207 of human MAP2K3 and MAP2K6; consider sequence alignment to confirm target specificity across species.
• For Western blot, use appropriate protein extraction buffers containing phosphatase inhibitors to preserve phosphorylation status, and validate band size (expected ~39 kDa for MAP2K3).
• In ELISA, ensure the plate coating peptide is phosphorylated; non-phosphorylated controls may be used to assess phosphorylation dependence.
• Optimal working dilutions must be empirically determined for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with integrated access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For targets like MAP2K3, involved in stress signaling pathways, we offer customized sourcing and technical support to streamline your assay development from concept to clinic. Partner with us for efficient procurement of specialized reagents.