Phospho-LCP2-Y145 Rabbit pAb for WB, ELISA - Q13094

Core Product Specifications and Parameters

Biological Background: LCP2 Function and Localization

• Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells. Related references: PMID:11313406, PMID:33159816
• Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Once phosphorylated by ZAP70, mediates interactions with VAV1, NCK and ITK. Related references: PMID:8673706, PMID:8702662
• Stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation. Related references: PMID:11313406
• Plays an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses. Related references: PMID:33436632
• Localizes to the cytoplasm.
• Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
• Contains an SH2 domain and is subject to phosphorylation; annotated as a phosphoprotein and reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y145; consider verifying specificity using a non-phosphorylated peptide control in parallel experiments.
• For Western blot, maintain phospho-protein integrity by including phosphatase inhibitors in lysis buffers and consider using a phospho-specific detection protocol.
• In ELISA, a sandwich approach with capture and detection antibodies may enhance signal; validate the assay with known phosphorylated and non-phosphorylated antigen standards.
• Cross-reactivity with mouse LCP2 is reported; confirm expected band size (~60 kDa) and sequence homology in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This phospho-LCP2-Y145 antibody supports immunology and inflammation research, enabling robust T-cell signaling studies. Explore our portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing solutions to accelerate your diagnostic development.