Phospho-Lck-Y505 Rabbit Polyclonal Antibody for WB, IP, ELISA - P06239

Core Product Specifications and Parameters

Biological Background: Lck Function and Localization

• Tyrosine-protein kinase Lck (p56-LCK) is a non-receptor tyrosine kinase of the Src family, essential for the selection and maturation of developing T-cells in the thymus and for mature T-cell function. Related references: PMID:2470098
• Lck plays a key role in T-cell antigen receptor (TCR)-linked signal transduction. It is constitutively associated with the cytoplasmic tails of CD4 and CD8 coreceptors, and upon TCR engagement, phosphorylates ITAM motifs in the TCR-CD3 complex, initiating signaling. Related references: PMID:2470098
• Once activated, Lck phosphorylates and activates the downstream kinase ZAP70, leading to the recruitment of signaling molecules and lymphokine production. Related references: PMID:2470098
• Lck also contributes to signaling via other receptors, including CD2-mediated hyperphosphorylation and the IL2 receptor pathway, which controls T-cell proliferation. Related references: PMID:2470098
• Additional substrates of Lck include RUNX3, PTK2B/PYK2, MAPT, RHOH, and TYROBP, and it interacts with FYB2. Related references: PMID:27335501
• Lck localizes to the cell membrane and cytoplasm (cytosol), and is expressed specifically in lymphoid cells.
• Post-translational modifications include N-terminal myristoylation and palmitoylation for membrane anchoring, and regulatory phosphorylation at key tyrosines, including Y505 (inhibitory). It contains SH2 and SH3 domains and is classified as a proto-oncogene and a kinase.

Experimental Guidance and Technical Tips

• The antibody is raised against a synthetic phosphopeptide corresponding to the Y505 region of human Lck; it is designed to specifically recognize Lck phosphorylated at Y505.
• For Western blot (WB), consider using cell or tissue lysates from human T-cell lines (e.g., Jurkat) with appropriate phosphatase inhibitors to preserve phosphorylation. A band around 58 kDa is expected.
• Immunoprecipitation (IP) may require optimization of antibody concentration and lysis buffer conditions; verify eluted proteins by WB.
• In ELISA, the antibody can be used to detect phospho-Lck-Y505 in peptide-based assays; plate coating and detection conditions should be empirically determined.
• Always validate the antibody in the relevant sample system and application context, as performance may vary with experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. For Lck-related research and T-cell signaling studies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material procurement. Contact our team to streamline your sourcing needs and accelerate your diagnostic development projects.