phospho-LATS1-S909/LATS2-S872 Rabbit pAb - O95835 / Q9NRM7

Core Product Specifications and Parameters

Biological Background: LATS1/LATS2 Function and Localization

• LATS1 is a serine/threonine-protein kinase that acts as a core tumor suppressor in the Hippo signaling pathway, restricting proliferation and promoting apoptosis to control organ size. Related references: PMID:10518011, PMID:10831611, PMID:18158288

• In the Hippo kinase cascade, STK3/MST2 and STK4/MST1, in complex with SAV1, phosphorylate and activate LATS1/2 in complex with MOB1; active LATS1 then phosphorylates YAP1 and WWTR1/TAZ, preventing their nuclear translocation and oncogenic activity. Related references: PMID:18158288, PMID:26437443, PMID:28068668

• Beyond Hippo signaling, LATS1 contributes to genomic stability by regulating mitotic progression and the G1 tetraploidy checkpoint, partly through down-regulating CDK1 kinase activity and modulating p53 expression. Related references: PMID:15122335, PMID:19927127, PMID:9988268

• LATS1 affects cytokinesis by negatively modulating LIMK1, thereby regulating actin polymerization. Related references: PMID:15220930

• In mammary gland epithelial cells, LATS1 promotes differentiation via both Hippo signaling and estrogen receptor signaling, including promoting the degradation of ESR1. Related references: PMID:28068668

• LATS1 functions as an activator of the NLRP3 inflammasome by mediating phosphorylation of NLRP3 at Ser-265, facilitated by NLRP3 palmitoylation and NEK7. Related references: PMID:39173637

• Subcellularly, LATS1 localizes to the centrosome, mitotic spindle, midbody, and spindle pole body during cell division.

• LATS1 is broadly expressed across adult tissues, with notably low or absent expression in lung and kidney.

Experimental Guidance and Technical Tips

• For phospho-specific western blot detection, always include phosphatase inhibitor cocktails during cell or tissue lysis to preserve labile phosphorylation signals.
• Validate antibody specificity by comparing signals from phosphatase-treated versus untreated lysates, and by peptide competition using the non-phosphorylated counterpart.
• Anticipate detection of phosphorylated LATS1 at ~126 kDa and phosphorylated LATS2 at ~76 kDa; confirm molecular weights with appropriate protein standards.
• For ELISA applications, optimize coating antigen and antibody dilution based on specific assay formats; the antibody may be used as a capture or detection reagent.
• As a rabbit polyclonal antibody, pair with anti-rabbit IgG secondary antibodies for signal detection; consider cross-adsorbed secondaries if multiplexing.
• Confirm reactivity in your human sample type of interest, as expression levels and phosphorylation status can vary among tissues and cell lines.

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CamelBio is your trusted one-stop sourcing partner for IVD raw materials, supplying high-quality antibodies like this phospho-LATS1-S909/LATS2-S872 pAb to advance Hippo pathway research and diagnostic assay development. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to meet your project needs, supporting you from concept to clinic.