Phospho-LAT-Y171 Rabbit pAb for WB and ELISA - O43561

Core Product Specifications and Parameters

Biological Background: LAT Function and Localization

• LAT (Linker for activation of T-cells) is a 36–38 kDa transmembrane adapter protein essential for coupling antigen receptor engagement to downstream signaling cascades. Related references: PMID:23514740 PMID:25907557
• It acts as a central scaffold upon TCR stimulation, recruiting PLCG1, GRB2, GRAP2, and other molecules to drive calcium flux, MAPK activation, and cytoskeletal reorganization.
• Beyond T-cells, LAT functions in natural killer cell FCGR3-mediated signaling and mast cell FCER1-mediated degranulation, highlighting its role in multiple immune effector pathways.
• The protein is localized to the cell membrane via a single transmembrane helix and undergoes lipid modifications including palmitoylation, which are critical for raft targeting and signaling microcluster formation.
• Extensive tyrosine phosphorylation—particularly at Y171—serves as a docking site for GRB2-family adapters, making it a key node in the TCR signalosome.
• In humans, LAT is predominantly expressed in thymus, T-cells, NK cells, and mast cells, with weak expression in spleen; it is undetectable in B-cells.

Experimental Guidance and Technical Tips

• This antibody was raised against a phosphorylated peptide spanning tyrosine 171; use appropriate phosphatase inhibitors during sample preparation to preserve the phospho-epitope.
• For western blot, include a non‑phospho peptide blocking control to confirm phospho‑specificity, and validate detection in stimulated vs. resting T‑cell lysates (e.g., Jurkat cells ± anti‑CD3).
• In ELISA, the synthetic phosphopeptide immunogen can serve as a solid‑phase antigen; consider using a non‑phosphorylated counterpart to subtract background reactivity.
• Cross‑reactivity with other species has not been established; validate reactivity in the intended sample system before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies validated raw materials—including phospho‑specific antibodies, matched pair reagents, and ancillary consumables—to support IVD manufacturers developing assays for T‑cell activation and immune signaling. As a dedicated sourcing partner, we streamline procurement of research‑grade and bulk‑grade anti‑LAT antibodies, along with complementary recombinant proteins, peptides, and detection reagents, to accelerate your diagnostic development from concept to clinic.