Phospho-Lamin A/C-S22 Rabbit pAb for WB, IP, ELISA - P02545

Core Product Specifications and Parameters

Biological Background: Prelamin-A/C Function and Localization

• Lamins are type V intermediate filament proteins that form the nuclear lamina, providing structural support to the nuclear envelope, organizing chromatin, and anchoring nuclear pore complexes, thereby playing critical roles in nuclear assembly and dynamics. Related references: PMID:10080180 PMID:10580070 PMID:10587585
• Lamin A and C regulate matrix stiffness by conferring mechanical properties to the nucleus, affecting cellular mechanotransduction. Related references: PMID:23990565 PMID:25127216
• The nuclear lamina undergoes cell-cycle-dependent disassembly and reassembly; phosphorylation at Ser22 is a key event driving lamina breakdown during mitosis, while dephosphorylation allows reassembly in telophase. Related references: PMID:2188730 PMID:2344612
• Lamin A/C is recruited to DNA double-strand breaks by XRCC4 and IFFO1, immobilizing broken DNA ends to prevent chromosome translocations. Related references: PMID:31548606
• Required for normal development of the peripheral nervous system and skeletal muscle, and for muscle satellite cell proliferation. Related references: PMID:10080180 PMID:10814726 PMID:11799477
• Essential for osteoblastogenesis and bone formation, and prevents fat infiltration of muscle and bone marrow. Related references: PMID:12075506 PMID:15317753 PMID:18611980 PMID:10587585
• In vascular smooth muscle cells, prelamin-A/C accumulation accelerates senescence by disrupting mitosis and inducing DNA damage. Related references: PMID:20458013
• Subcellular localization: Found in the nuclear lamina, nuclear envelope, nucleoplasm, nuclear matrix, and nuclear speckles.
• Tissue specificity: In arteries, prelamin-A/C expression increases with age and disease, and colocalizes with senescent VSMCs in atherosclerotic lesions.

Experimental Guidance and Technical Tips

• Since the immunogen is a phosphorylated peptide, the antibody specifically recognizes phosphorylation at Ser22; use phosphatase inhibitors during sample preparation to preserve this modification.
• For Western blotting, a protein band of approximately 74 kDa is expected; consider using a 8–12% SDS-PAGE gel for optimal separation and confirm with appropriate molecular weight markers.
• Given the cell-cycle-dependent phosphorylation of lamins, the detection signal may vary; consider synchronizing cells or collecting samples at defined mitotic stages to maximize signal.
• The antibody is validated for human and rat, but cross-reactivity with other species should be checked experimentally; for IP, testing under both native and denaturing conditions is recommended.
• As a rabbit polyclonal antibody, it may exhibit lot-to-lot variability; always titrate and validate in your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-quality IVD raw materials for diagnostic developers and research groups worldwide. This anti-phospho-Lamin A/C-S22 rabbit polyclonal antibody supports cutting-edge studies in nuclear lamina dynamics, laminopathies, and cell cycle control. Our integrated supply chain offers validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials to simplify your assay development—from concept to clinic.