Phospho-JunB-S259 Rabbit Polyclonal Antibody for WB - P17275

Core Product Specifications and Parameters

Biological Background: JunB Function and Localization

• JunB is a transcription factor (also known as AP-1 subunit JunB) that binds to the DNA consensus sequence 5'-TGA[GC]TCA-3' to regulate gene expression following growth factor stimulation.
• It heterodimerizes with FOS family proteins to form the AP-1 transcription complex, significantly enhancing its DNA binding affinity and transcriptional activity.
• JunB functions primarily in the nucleus, where it participates in transcriptional regulation.
• The protein is subject to multiple post-translational modifications, including phosphorylation (as a phosphoprotein), acetylation, and ubiquitin-like conjugation (Ubl conjugation), which may modulate its activity.
• UniProt annotates JunB with keywords such as DNA-binding, isopeptide bond formation, and reference proteome status, indicating broad research interest.
• The calculated molecular weight of JunB is approximately 35.9 kDa, and it migrates around 36 kDa in SDS-PAGE (as confirmed by the antibody's target).

Experimental Guidance and Technical Tips

• This antibody was raised against a phospho-specific peptide corresponding to residues surrounding Ser259 of human JunB; thus, it is expected to specifically recognize JunB phosphorylated at S259.
• For WB applications, consider using phosphatase inhibitors during sample preparation to preserve phosphorylation signals, and validate antibody specificity via phosphatase treatment or non-phosphorylated peptide blocking.
• Given cross-reactivity with human, mouse, and rat, the antibody is suitable for comparative studies across these species; however, confirm performance in each species with appropriate controls.
• Because JunB is a transcription factor, nuclear extracts or whole-cell lysates may be required for detection; optimize lysis and loading conditions accordingly.
• Note that the predicted molecular weight of JunB is ~36 kDa; any observed band shift might indicate post-translational modifications or isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting. For transcription factor and AP-1 signaling research, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our integrated platform streamlines procurement from concept to clinical application.