Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit pAb - P45983 / P45984 / P53779

Core Product Specifications and Parameters

Biological Background: JNK1/2/3 (MAPK8/9/10) Function and Localization

• Mitogen-activated protein kinase 8 (MAPK8, also known as JNK1, SAPK1c) is a serine/threonine-protein kinase belonging to the JNK family, which includes JNK2 (MAPK9) and JNK3 (MAPK10).
• MAPK8 is a core component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) pathway, which is stimulated by pro-inflammatory cytokines and cellular stress. Upon activation by MAP2K4 and MAP2K7, MAPK8 phosphorylates transcription factors such as JUN and ATF2, thereby regulating AP-1 transcriptional activity (PubMed:28943315, PubMed:18307971). Related references: PMID:28943315, PMID:18307971
• MAPK8 promotes apoptosis under stress conditions by phosphorylating key regulatory proteins including p53/TP53 and YAP1 (PubMed:21364637). Related references: PMID:21364637
• In nutrient-deprived cells, MAPK8 phosphorylates BCL2, leading to its dissociation from BECN1 and induction of autophagy (PubMed:18570871). Related references: PMID:18570871
• MAPK8 participates in circadian rhythm regulation by phosphorylating the CLOCK-BMAL1 heterodimer (PubMed:22441692). Related references: PMID:22441692
• Subcellularly, MAPK8 localizes to the cytoplasm, nucleus, and synapse, consistent with its roles in signal transduction and neuronal function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide spanning Thr183 and Tyr185 of human JNK1; these residues are conserved in JNK2 (Thr183/Tyr185) and JNK3 (Thr221/Tyr223), suggesting potential cross-reactivity with all three family members.
• For Western blot (WB), expect multiple bands corresponding to different JNK isoforms and phosphorylation states; consider validating band identity using phosphatase treatment or specific siRNAs.
• The antibody is suitable for ELISA; use non-phosphorylated peptide controls to confirm phospho-specificity.
• Cross-reactivity with human and mouse supports use in comparative studies, but always validate performance in the exact experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner offering one-stop access to validated antibodies, antibody pairs, and ancillary reagents for diagnostic and research applications. With expertise in stress kinase signaling, we supply optimized rabbit polyclonal antibodies, monoclonal antibodies, and bulk raw materials to streamline assay development—from concept to clinic. Explore our catalog for rare-target sourcing and tailored support in molecular biology research.