Phospho-JAK2-Y1008 Rabbit pAb for WB, ELISA - O60674

Core Product Specifications and Parameters

Biological Background: JAK2 Function and Localization

• Protein identity and family: Tyrosine-protein kinase JAK2 (Janus kinase 2) is a non-receptor protein tyrosine kinase that plays a central role in cytokine and growth factor signaling. It contains a kinase domain, a pseudokinase domain, and an SH2 domain, and is activated by autophosphorylation at key residues including Y1008. Related references: PMID:7615558
• Signal transduction mechanism: Upon ligand binding to type I or type II cytokine receptors, JAK2 phosphorylates specific tyrosine residues on the receptor cytoplasmic tails, creating docking sites for STAT transcription factors. JAK2 then phosphorylates STATs, which dimerize and translocate to the nucleus to regulate gene expression. Related references: PMID:15690087, PMID:9618263
• Erythropoiesis regulation: JAK2 is essential for erythropoietin (EPO) signaling. EPO binding induces JAK2 autophosphorylation and activation, leading to EPOR phosphorylation, STAT5 recruitment, and activation of genes required for erythropoiesis. Related references: PMID:9657743
• Chromatin modifier: In the nucleus, JAK2 phosphorylates histone H3 at tyrosine-41 (H3Y41ph), which displaces heterochromatin protein HP1α (CBX5) from chromatin, thereby influencing gene expression and chromatin structure. Related references: PMID:19783980
• Subcellular localization: JAK2 is found in the cytoplasm, associated with the endomembrane system, and in the nucleus, reflecting its multiple roles in signal transduction and nuclear events.
• Ubiquitous expression: JAK2 is ubiquitously expressed across most tissues, consistent with its fundamental roles in cell growth, differentiation, and immunity.
• Other key interactions: JAK2 cooperates with TEC kinase, modulates CDKN1B to regulate cell cycle, and participates in oncostatin-M and CNTF signaling pathways. Related references: PMID:21423214, PMID:9188471, PMID:11294841
• Disease relevance: As a proto-oncogene, JAK2 gain-of-function mutations (e.g., V617F) are associated with myeloproliferative disorders. Keywords also link it to adaptive and innate immunity, chromosomal rearrangement, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide encompassing the Y1008 autophosphorylation site. This site is critical for JAK2 kinase activation; ensure that detection reflects the phosphorylation status of your target by including appropriate controls (e.g., λ‑phosphatase treatment).
• Western blot is recommended; the predicted molecular weight of JAK2 is 131 kDa. Use a fresh blocking buffer and verify band specificity by competition with the non‑phosphorylated peptide.
• Confirm cross‑reactivity with your specific human sample system. For ELISA, consider coating with the phosphopeptide and validating with known JAK2‑activated cell lysates.
• Avoid repeated freeze‑thaw cycles and store as recommended (ice bag shipping) to preserve antibody activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in empowering diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, from validated monoclonal and polyclonal antibodies to bulk ancillary reagents. This phospho‑specific JAK2 antibody supports targeted research in signal transduction, myeloproliferative disorders, and chromatin biology. Through integrated sourcing, technical consultancy, and custom development support, CamelBio helps streamline your journey from concept to clinic.