Phospho-JAK2-Y1007/1008 Rabbit mAb for WB, ELISA - O60674

Core Product Specifications and Parameters

Biological Background: JAK2 Function and Localization

• JAK2 is a non-membrane spanning protein tyrosine kinase that phosphorylates type I cytokine receptors (e.g., GHR, EPOR, MPL) and type II receptors (e.g., IFN receptors, interleukins), initiating downstream signaling cascades. Related references: PMID:15690087 PMID:15899890 PMID:7615558
• Mediates essential signaling in innate and adaptive immunity, and is involved in processes such as cell growth, development, differentiation, and histone modifications.
• Upon ligand binding, JAK2 phosphorylates specific tyrosine residues on the cytoplasmic receptor tails, creating docking sites for STAT proteins; it then phosphorylates STATs, which dimerize and translocate to the nucleus to regulate gene transcription. Related references: PMID:9618263
• In erythropoiesis, EPO stimulation leads to JAK2 autophosphorylation and activation, association with EPOR, and subsequent phosphorylation and activation of STAT5, which promotes transcription of erythropoiesis-related genes. Related references: PMID:9657743
• In the nucleus, JAK2 phosphorylates histone H3 at Tyr-41 (H3Y41ph), a modification that excludes HP1α from chromatin, thereby playing a direct role in epigenetic regulation. Related references: PMID:19783980
• Subcellular localization includes the endomembrane system, cytoplasm, and nucleus, consistent with its dual cytoplasmic signaling and nuclear functions.
• Ubiquitously expressed across most tissues, reflecting its broad role in cytokine signaling.
• Key structural features include an SH2 domain and tandem kinase-like domains; classified as a tyrosine-protein kinase, chromatin regulator, and proto-oncogene.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphorylated peptide spanning Y1007 and Y1008; it is expected to specifically recognize JAK2 only when phosphorylated at these activation loop sites.
• For WB, consider using phosphatase inhibitor cocktails during sample preparation and running appropriate positive/negative controls (e.g., stimulated vs. unstimulated cells) to confirm phospho-specificity.
• The predicted molecular weight of JAK2 is ~131 kDa; verify band migration against molecular weight markers and account for possible post‑translational modifications.
• Pre‑treatment of cell lysates with lambda phosphatase can serve as a useful control for determining phospho‑specificity.
• For ELISA, follow standard immobilization and detection protocols; pairing with a total JAK2 antibody may allow normalization of phosphorylation levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to IVD raw materials, including phospho-specific antibodies for signal transduction targets like JAK2. With validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and the capacity to source rare-target materials, we support your workflow from concept to clinic. Whether you need robust JAK2 phospho‑detection reagents or comprehensive signaling panel development, CamelBio is your trusted partner in IVD raw-material supply.