Phospho-IRS1-S612/S636 Rabbit mAb for WB, IHC-P, ELISA - P35568

Core Product Specifications and Parameters

Biological Background: Insulin receptor substrate 1 Function and Localization

• IRS1 is a signaling adapter protein that mediates signal transduction from the insulin receptor (INSR) and insulin-like growth factor I receptor (IGF1R). Related references: PMID:7541045, PMID:33991522, PMID:38625937
• It plays a critical role in the regulation of development, growth, glucose homeostasis, and lipid metabolism. Related references: PMID:19639489
• Upon insulin stimulation, IRS1 is phosphorylated by the insulin receptor and serves as a scaffold for SH2 domain-containing proteins, including p85 PI3K, GRB2, NCK1, NCK2, and SHP2. Related references: PMID:11171109, PMID:8265614
• IRS1 promotes Wnt/β-catenin signaling by suppressing autophagy-mediated degradation of DVL2, leading to enhanced cell proliferation. Related references: PMID:24616100
• Subcellular localization includes both cytoplasm and nucleus, enabling diverse signaling functions.
• Post-translational modifications such as phosphorylation and ubiquitination regulate IRS1 activity and stability; genetic variants are associated with diabetes mellitus.

Experimental Guidance and Technical Tips

• For WB, use insulin-stimulated cell lysates as positive controls to induce IRS1 phosphorylation at S612/S636. Target band expected around 132 kDa.
• For IHC-P, consider antigen retrieval protocols optimized for phospho-specific antibodies and validate with relevant tissue sections.
• The immunogen was designed around S616, but the antibody detects phosphorylation at S612/S636. Verify cross-reactivity and specificity with appropriate phospho-peptide blocking experiments.
• When performing ELISA, pair with a total IRS1 antibody to normalize phosphorylated signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target development. The Phospho-IRS1-S612/S636 Rabbit mAb exemplifies our commitment to providing high-quality reagents for insulin signaling and metabolic disease research, supporting your diagnostic and translational workflows from concept to clinic.