Phospho-IRS1-S312 Rabbit Polyclonal Antibody for Western Blot - P35568

Core Product Specifications and Parameters

Biological Background: IRS1 Function and Localization

• Insulin receptor substrate 1 (IRS1) is a critical signaling adapter protein that mediates signal transduction from insulin receptor (INSR) and insulin-like growth factor I receptor (IGF1R), playing essential roles in development, growth, glucose homeostasis, and lipid metabolism. Related references: PMID:7541045, PMID:19639489
• Upon phosphorylation by activated insulin receptor, IRS1 serves as a docking/scaffold protein, recruiting SH2 domain-containing proteins such as the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2, and SHP2 to propagate downstream signals. Related references: PMID:11171109, PMID:8265614
• Recruitment of GRB2-SOS1 complex leads to activation of the Ras/Raf/MEK/MAPK cascade, while PI3K/AKT pathway activation mediates most of insulin's metabolic effects; both pathways cooperate to regulate gene expression, cell growth, and differentiation.
• IRS1 also acts as a positive regulator of the Wnt/β-catenin signaling pathway by suppressing autophagy-mediated degradation of DVL2, thereby promoting cell proliferation. Related references: PMID:24616100
• IRS1 localizes to both the cytoplasm and nucleus, consistent with its roles in signaling and gene regulation.
• The protein is subject to extensive post-translational modifications including phosphorylation, ubiquitination, and S-nitrosylation, which finely tune its activity and stability; keywords include Phosphoprotein, Ubl conjugation, and Diabetes mellitus.
• Mutations or dysregulation of IRS1 are associated with insulin resistance and type 2 diabetes, underscoring its importance in metabolic disease.
• Human IRS1 has a calculated molecular weight of about 131.6 kDa and migrates at approximately 132 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen is a phospho-specific peptide corresponding to residues surrounding serine 312 of human IRS1. Specificity for the phosphorylated form should be confirmed; consider using phosphatase-treated lysates or non-phospho peptide blocking in your validation.
• Western blot detection of IRS1 typically yields a band around 132 kDa; use appropriate molecular weight markers and consider the protein's known migration behavior.
• For phosphorylated IRS1, treatment of cells with insulin or IGF-1 prior to lysis is expected to enhance the signal; optimize stimulation time and concentration for your system.
• This antibody shows cross-reactivity with mouse and rat IRS1 based on supplier data; validate in the relevant sample system using appropriate controls.
• Given the susceptibility of phospho-epitopes, avoid harsh stripping and reprobing of membranes; if necessary, verify that the phospho-signal is not lost.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with reliable access to IVD raw materials, including validated phospho-specific antibodies like this anti-phospho-IRS1 (Ser312) product for insulin signaling research. We offer a comprehensive selection of antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials to streamline your assay development from concept to clinic. With our integrated supply capabilities, you can confidently bridge the gap between research and diagnostic application.