Phospho-IRS1-S307(Human Specific) Rabbit pAb for WB, IHC-P, ELISA - P35568

Core Product Specifications and Parameters

Biological Background: Insulin receptor substrate 1 Function and Localization

• Insulin receptor substrate 1 (IRS1) is a critical signaling adapter that relays signals from the insulin receptor (INSR) and insulin-like growth factor 1 receptor (IGF1R) to downstream pathways, playing a key role in glucose homeostasis and growth (PubMed:7541045, 33991522, 38625937). Related references: PMID:7541045 PMID:33991522 PMID:38625937

• Upon phosphorylation, IRS1 acts as a docking scaffold for SH2 domain-containing proteins such as the p85 subunit of PI3K, GRB2, and SHP2, thereby initiating the PI3K/AKT and Ras/MAPK signaling cascades (PubMed:11171109, 8265614). Related references: PMID:11171109 PMID:8265614

• IRS1 positively modulates the Wnt/β-catenin pathway by preventing autophagy-mediated degradation of DVL2, which promotes cell proliferation (PubMed:24616100). Related references: PMID:24616100

• The PI3K/AKT arm of IRS1 signaling is central to insulin's metabolic effects, including glucose uptake and glycogen synthesis, whereas the Ras/MAPK branch regulates gene expression and cell growth.

• IRS1 is predominantly localized to the cytoplasm but can also be detected in the nucleus, where it may associate with nuclear proteins to influence transcription.

• As a highly regulated phosphoprotein, IRS1 undergoes serine/threonine phosphorylation at multiple sites; phosphorylation at S307 in humans is believed to modulate insulin signal duration and sensitivity.

• Aberrant IRS1 function is implicated in insulin resistance, type 2 diabetes, and related metabolic disorders.

Experimental Guidance and Technical Tips

• Since the antibody targets the phosphorylation site S307, it is crucial to use phosphatase inhibitors during sample preparation to preserve phospho-signals.
• For Western blot, consider optimizing antibody dilution and verifying specificity with non-phosphorylated peptide blocking or phosphatase-treated lysates.
• In IHC-P, standard heat-induced antigen retrieval may be employed; validate staining patterns in positive and negative tissue controls.
• Cross-reactivity with mouse and rat IRS1 is expected due to high sequence homology around S307, but confirm specificity in your model system.
• For ELISA applications, ensure that the coating antigen includes the phosphorylated epitope and consider a capture/detection format.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, from validated antibodies and antigen pairs to bulk ancillary reagents. This phospho-specific IRS1 S307 antibody supports insulin signaling research and metabolic disease assay development, and our team can further assist with custom sourcing, technical consulting, and supply chain solutions to accelerate your project from concept to clinic.