Phospho-IRS1-S1101 Rabbit pAb for WB, ELISA - P35568

Core Product Specifications and Parameters

Biological Background: IRS1 (Insulin Receptor Substrate 1) Function and Localization

• IRS1 is a signaling adapter protein that mediates signal transduction from the insulin receptor (INSR) and insulin-like growth factor I receptor (IGF1R), playing a central role in development, growth, glucose homeostasis, and lipid metabolism.
  Related references:
  PMID:7541045, PMID:33991522, PMID:38625937, PMID:19639489

• Upon phosphorylation by activated insulin receptor, IRS1 serves as a scaffold for SH2 domain-containing proteins such as the p85 subunit of PI3K, GRB2, NCK1, NCK2, and SHP2, thereby coupling receptor activation to downstream cascades.
  Related references:
  PMID:11171109, PMID:8265614

• Through GRB2 recruitment, IRS1 triggers the Ras/Raf/MEK/MAPK signaling module, which regulates gene expression, cell growth, and differentiation.
  Related references:
  PMID:7541045 (By similarity)

• Activation of the PI3K/AKT pathway via IRS1 is responsible for most of the metabolic effects of insulin, including glucose uptake and glycogen synthesis.
  Related references:
  PMID:11171109

• IRS1 also functions as a positive regulator of the Wnt/β-catenin pathway by suppressing DVL2 autophagy-mediated degradation, thereby promoting cell proliferation.
  Related references:
  PMID:24616100

• Subcellularly, IRS1 is found in both the cytoplasm and nucleus, consistent with its roles in rapid signaling and transcriptional regulation.

• IRS1 is subject to extensive phosphorylation, S-nitrosylation, and ubiquitination, with phosphorylation at Ser1101 being one of the regulatory modifications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide matching the region around Ser1101 of human IRS1; consider using this antibody to specifically detect phosphorylation at this site in response to insulin or IGF-1 stimulation.
• For Western blot, validate in appropriate human or mouse cell lines (e.g., HepG2, 3T3‑L1 adipocytes) treated with insulin; a band around 132 kDa is expected.
• Ser1101 phosphorylation may be transient or stimulus-dependent; include appropriate controls (e.g., unstimulated samples, phosphatase treatment) to confirm signal specificity.
• This antibody is suitable for ELISA; consider using a capture-detection pair design with a total IRS1 antibody for quantitative phospho‑protein measurement.
• Given the cross-reactivity with mouse, the antibody can be applied to mouse models of insulin resistance or diabetes for translational studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutions with reliable access to high-quality IVD raw materials. This phospho‑IRS1‑S1101 antibody supports advanced insulin signaling and metabolic disease research, and can be integrated into customized immunoassay development. Beyond this specialized reagent, CamelBio offers validated antibody pairs, bulk ancillary reagents, and rare‑target sourcing services to streamline your entire assay development pipeline—from concept to clinical application.