Phospho-IRF3-S386 Rabbit pAb for WB and ELISA - Q14653

Core Product Specifications and Parameters

Biological Background: IRF3 Function and Localization

• Key transcriptional regulator of type I interferon (IFN)-dependent immune responses, controlling expression of IFN-α/β and IFN-stimulated genes (ISGs) by binding to interferon-stimulated response element (ISRE) promoters.
  Related references: PMID:11846977, PMID:16846591, PMID:16979567, PMID:20049431

• Exists in an inactive monomeric form in the cytoplasm of uninfected cells; upon viral infection or TLR signaling, kinases IKBKE and TBK1 phosphorylate IRF3 at key residues including S386, inducing conformational change, dimerization, and nuclear translocation.
  Related references: PMID:22394562, PMID:25636800, PMID:27302953

• In the nucleus, phosphorylated IRF3 associates with CREBBP to form the dsRNA-activated factor 1 (DRAF1) complex, which potently activates transcription of type I IFN and ISG genes.
  Related references: PMID:16154084, PMID:33440148

• Plays a critical role in both early and late phases of IFN gene induction, and can activate distinct gene expression programs in macrophages, including the ability to induce significant apoptosis in primary macrophages.
  Related references: PMID:16846591, PMID:25609812

• Key transcription factor in the IFN response during SARS-CoV-2 infection, mediating antiviral defense.
  Related references: PMID:33440148

• Subcellular localization shuttles between cytoplasm, nucleus, and mitochondrion; mitochondrial recruitment (via TOMM70:HSP90AA1) can trigger apoptosis through BAX.
  Related references: PMID:25609812

• Constitutively expressed across a wide range of tissues; gene undergoes alternative splicing and the protein is subject to acetylation, phosphorylation, and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to the S386 phosphorylation site; we recommend using phosphatase inhibitors during sample lysis to preserve the phosphorylation signal.
• For Western blot, a band around 47 kDa is expected; include a non‑phospho‑peptide blocking control to confirm phospho‑specificity.
• In ELISA, direct coating with the phospho‑peptide or capture of the target from lysates may be used; titration of the antibody is advised for optimal signal‑to‑noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, supporting diagnostic developers from early R&D through clinical application. This anti‑phospho‑IRF3‑S386 antibody is part of our extensive portfolio for innate immunity and infectious disease research. Beyond individual antibodies, we provide validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and access to rare‑target sourcing—all designed to streamline your assay development and supply chain.