Phospho-IRE1-S724 Rabbit pAb - O75460

Core Product Specifications and Parameters

Biological Background: IRE1 Function and Localization

• IRE1 (also known as ERN1, IRE1α) is a dual-function serine/threonine-protein kinase and endoribonuclease that acts as a primary sensor for the endoplasmic reticulum unfolded protein response (UPR). Related references: PMID:11175748, PMID:11779464, PMID:12637535
• In unstressed cells, IRE1 is maintained in an inactive monomeric state through binding to the ER chaperone HSPA5/BiP. Related references: PMID:21317875
• Upon accumulation of misfolded proteins, HSPA5/BiP dissociates, allowing IRE1 homodimerization and trans-autophosphorylation of the kinase domain, which activates the endoribonuclease function. Related references: PMID:21317875
• The endoribonuclease domain specifically cleaves XBP1 mRNA, excising a 26-nucleotide intron to generate spliced XBP1 (XBP1s). Related references: PMID:11779464, PMID:21317875, PMID:24508390
• XBP1s encodes a potent transcription factor that upregulates genes involved in ER protein folding, quality control, and degradation, restoring ER homeostasis. Related references: PMID:11779464, PMID:21317875, PMID:24508390
• Beyond XBP1 splicing, IRE1 also regulates unconventional protein secretion pathways, such as ER stress-induced trafficking of CFTR to the cell membrane via GORASP2 and SEC16A. Related references: PMID:21884936, PMID:28067262
• IRE1 is ubiquitously expressed, with particularly high levels in pancreatic tissue, reflecting the high secretory demand of pancreatic cells.
• As an endoplasmic reticulum membrane protein, IRE1's N-terminal luminal domain senses ER stress, while its cytosolic C-terminal region houses the kinase and endoribonuclease activities.

Experimental Guidance and Technical Tips

• This antibody is raised against a phosphorylated peptide corresponding to the S724 region of human IRE1α. To verify phospho-specificity, consider λ-phosphatase treatment of samples prior to Western blot analysis.
• For WB, IRE1 migrates at approximately 110 kDa. Ensure thorough membrane blocking and include appropriate positive (ER-stressed cell lysates) and negative (unstressed cells) controls to confirm band identity.
• In IHC-P, antigen retrieval conditions may require optimization. Include an isotype control and validate staining patterns against known IRE1 tissue distribution.
• Cross-reactivity with mouse and rat IRE1 is reported; however, alignment of the epitope sequence around S724 is recommended to confirm conservation in these species.
• As a rabbit polyclonal antibody, lot-to-lot performance can vary. Validate each new lot in your specific assay system to ensure consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate diagnostic development. With this Phospho-IRE1-S724 Rabbit pAb, researchers gain a key reagent for studying ER stress and the unfolded protein response. CamelBio further supports your projects with tailored rare-target sourcing and technical consulting from concept to clinic.