Phospho-IRE1-S724 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O75460

Core Product Specifications and Parameters

Biological Background: IRE1 Function and Localization

• IRE1 (ERN1) is a bifunctional enzyme combining a serine/threonine kinase domain and an endoribonuclease domain, serving as a master sensor of ER stress and the unfolded protein response (UPR). Related references: PMID:11175748, PMID:11779464, PMID:12637535
• Under basal conditions, IRE1 monomers are kept inactive by binding to the ER chaperone BiP/HSPA5. Accumulation of misfolded proteins triggers BiP release, permitting IRE1 homodimerization and trans-autophosphorylation. Related references: PMID:21317875
• Activated IRE1 endoribonuclease specifically targets XBP1 mRNA, excising a 26-nucleotide intron to produce spliced XBP1 (XBP1s). Related references: PMID:11779464, PMID:21317875, PMID:24508390
• Spliced XBP1 encodes a potent transcription factor that drives expression of UPR target genes, promoting protein folding and ER-associated degradation. Related references: PMID:11779464, PMID:21317875, PMID:24508390
• IRE1 also mediates ER stress-induced, GORASP2-dependent unconventional trafficking of CFTR to the plasma membrane, involving SEC16A redistribution. Related references: PMID:21884936, PMID:28067262
• Immunofluorescence studies localize IRE1 predominantly to the endoplasmic reticulum membrane.
• IRE1 is ubiquitously expressed, with particularly high levels in pancreatic tissue.

Experimental Guidance and Technical Tips

• For phospho-specific detection in western blotting, include phosphatase inhibitors in lysis buffers to preserve the Ser724 phosphorylation signal.
• Employ a non-phospho IRE1 antibody as a loading control to normalize phospho- and total protein levels.
• The predicted molecular weight is approximately 110 kDa; use appropriate molecular weight markers for accurate size confirmation.
• For IHC-P and IF/ICC, antigen retrieval and permeabilization conditions may require optimization for different sample types.
• Although the antibody is designated for WB, IHC-P, IF/ICC, and ELISA, optimal dilutions and incubation times should be experimentally determined in the relevant model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated phospho-specific antibodies for ER stress and UPR pathway targets like IRE1. Our one-stop sourcing streamlines procurement of matched antibody pairs, bulk ancillary reagents, and rare-target raw materials, supporting seamless transition from concept to clinic.