Phospho-IRAK4-Ser346 Rabbit mAb for WB, ELISA - Q9NWZ3

Core Product Specifications and Parameters

Biological Background: IRAK4 Function and Localization

• IRAK4 (Interleukin-1 receptor-associated kinase 4), also known as renal carcinoma antigen NY-REN-64, is encoded by the IRAK4 gene. Multiple isoforms exist due to alternative splicing.
• IRAK4 is a serine/threonine kinase essential for initiating innate immune responses against pathogens. It functions downstream of Toll-like receptors (TLRs) and IL-1R, being rapidly recruited to the Myddosome complex via MYD88. Related references: PMID:17878374
• Upon activation, IRAK4 phosphorylates IRAK1, stimulating its kinase activity and autophosphorylation. It also phosphorylates Pellino E3 ubiquitin ligases (PELI1, PELI2, PELI3) to promote K63-linked polyubiquitination of IRAK1, leading to TAK1 and IKK complex activation and NF-κB nuclear translocation.
• IRAK4 also phosphorylates TIRAP to promote its ubiquitination and degradation, and phosphorylates NCF1 to regulate NADPH oxidase activity during LPS stimulation, contributing to antimicrobial responses.
• Predominantly localized in the cytoplasm.
• IRAK4 undergoes phosphorylation and acetylation, which are critical for its activation and function.
• Mutations in IRAK4 are associated with immunodeficiency disorders (e.g., IPD1, IMD67) and renal carcinoma (NY-REN-64), highlighting its role in immune defense and cancer.

Experimental Guidance and Technical Tips

• Since this antibody is specific for phosphorylated Ser346, include phosphatase inhibitors during sample preparation for western blotting to preserve the phosphorylation signal.
• The immunogen is a synthetic phosphopeptide; consider using a non‑phosphorylated peptide as a negative control to confirm phosphorylation‑dependent binding.
• For western blotting, the predicted molecular weight is approximately 52 kDa. Ensure adequate protein loading and use appropriate positive controls, such as stimulated immune cells.
• For ELISA applications, develop a phospho‑specific detection protocol; pairing with a total IRAK4 antibody may allow ratiometric analysis of phosphorylation levels.
• Validate the antibody in the relevant human sample system, as cross‑reactivity with other species has not been determined.
• Optimize antibody dilution and incubation conditions for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one‑stop sourcing partner for IVD raw materials, offering validated antibodies, matched pairs, and bulk reagents to support diagnostic manufacturers and research laboratories. For innate immunity and kinase targets such as phospho‑IRAK4, we provide optimized monoclonal antibodies, ancillary reagents, and rare‑target raw‑material sourcing to streamline assay development and production. Our supply capabilities cover every stage from concept to clinic, ensuring reliable and quality‑assured materials for your critical workflows.