Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb for WB, ELISA - P08069/P06213

Core Product Specifications and Parameters

Biological Background: IGF1R (Insulin-like Growth Factor 1 Receptor) Function and Localization

• IGF1R, alternatively named insulin-like growth factor I receptor (IGF-I receptor), is a receptor tyrosine kinase that binds IGF1 with high affinity and IGF2 and insulin with lower affinity.
• Ligand binding induces autophosphorylation and phosphorylation of substrates like IRS1/2 and Shc, activating the PI3K-AKT and Ras-MAPK pathways to promote cell growth, survival, and proliferation.
• IGF1R can form hybrid receptors with INSR; hybrids with INSR Long bind IGF1 with high affinity and not insulin (PubMed:12138094), but contrasting data suggest similar binding characteristics for Long and Short hybrids (PubMed:16831875).
  Related references: PMID:12138094, PMID:16831875
• Additional signaling through JAK/STAT (notably STAT3) and JNK pathways further mediates IGF1R’s mitogenic and antiapoptotic effects.
• The receptor is localized to the cell membrane and is overexpressed in various tumors (melanoma, colon, pancreatic, prostate, and kidney cancers).
• IGF1R is widely expressed, and hybrid INSR-IGF1R receptors are detected in muscle, heart, kidney, adipose, liver, fibroblasts, spleen, and placenta.
• The mature receptor is a disulfide-linked heterotetramer generated by proteolytic cleavage of a precursor, and it is heavily glycosylated and phosphorylated upon activation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Y1165/Y1166 of human IGF1R/INSR, suggesting specificity for dual phosphorylated activation loop sites; this antibody may be useful for detecting activated receptor signaling.
• For Western blotting, consider treating cells with IGF1 or insulin before lysis to induce receptor phosphorylation; include phosphatase inhibitors in lysis buffer to preserve phospho-epitopes.
• As a rabbit polyclonal, this antibody may detect both phospho‑IGF1R and phospho‑INSR; verify cross‑reactivity in relevant species (human, mouse, rat) and consider isoform‑specific controls.
• ELISA suitability implies potential for quantitative signaling assays; optimize coating conditions and verify specificity with non‑phosphorylated peptide controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop sourcing partner for IVD raw materials, providing validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This phospho‑specific antibody supports research and diagnostic development in insulin/IGF signaling and related metabolic disease areas. Contact us to source bulk quantities or specialized raw materials for your assay needs.