Phospho-IKKγ (S376) Rabbit Polyclonal Antibody for WB, ELISA - Q9Y6K9

Core Product Specifications and Parameters

Biological Background: IKKγ (NEMO) Function and Localization

• IKKγ (NEMO, IKBKG) is the regulatory subunit of the IKK core complex, which phosphorylates NF-kB inhibitors, leading to inhibitor degradation and NF-kB activation. Related references: PMID:14695475, PMID:20724660, PMID:21518757
• It binds scaffolding polyubiquitin chains, recognizing both 'Lys-63'-linked and linear polyubiquitin with high affinity for linear chains, playing a key role in IKK activation by multiple signaling receptors. Related references: PMID:16547522, PMID:18287044, PMID:19033441
• IKKγ is implicated in NF-kB-mediated protection from cytokine toxicity and is essential for viral activation of IRF3. Related references: PMID:19854139
• Involved in antiviral innate immune responses triggered by TLR3 and IFIH1, requiring 'Lys-27'-linked polyubiquitination. Related references: PMID:20724660
• Also functions as a mediator for HTLV-1 Tax oncoprotein activation of NF-kB.
• Subcellular localization includes both the cytoplasm and nucleus.
• Expressed in a wide range of tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Contains a coiled coil domain and a zinc finger domain; mutations in the gene are associated with ectodermal dysplasia and osteopetrosis.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Ser376 of human IKKγ. For optimal phospho-specific detection, include phosphatase inhibitors in lysis buffers and consider using phosphatase treatment as a negative control.
• The antibody has been validated for Western blot (WB) and ELISA; for other applications, we recommend pilot experiments to determine appropriate dilutions and conditions.
• Predicts bands at approximately 48 kDa, 56 kDa, and 37 kDa; multiple bands may reflect alternative splicing or post-translational modifications.
• Expected to cross-react with human, mouse, and rat IKKγ based on sequence homology; for other species, verify epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering ready-to-use phospho-specific antibodies like this anti-phospho-IKKγ (S376) antibody, ideal for NF-kB pathway research. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development. Our team also assists with sourcing rare-target raw materials to streamline your assay development pipeline.