Phospho-IκBα-S36 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P25963

Core Product Specifications and Parameters

Biological Background: IκBα Function and Localization

• IκBα (NF-kappa-B inhibitor alpha, also known as MAD3 or NFKBI) is a key regulatory protein that inhibits NF-κB transcription factors by retaining RELA/p65 and NFKB1/p50 dimers in the cytoplasm through masking their nuclear localization signals. Related references: PMID:1493333 PMID:36651806 PMID:7479976

• Upon cellular stimulation by immune and pro-inflammatory signals, IκBα undergoes phosphorylation at Ser32 and Ser36, which triggers its ubiquitination and subsequent proteasomal degradation, thereby releasing NF-κB dimers to translocate to the nucleus and activate transcription. Related references: PMID:7479976 PMID:7628694 PMID:7796813

• IκBα is a member of the IκB family of inhibitors, characterized by ankyrin repeats that mediate protein-protein interactions with NF-κB dimers.

• Subcellularly, IκBα shuttles between the cytoplasm and the nucleus, but its predominant localization is cytoplasmic where it sequesters inactive NF-κB complexes.

• Post-translational modifications of IκBα include phosphorylation, ubiquitination (Ubl conjugation), hydroxylation, and isopeptide bond formation, which regulate its stability and activity.

• The gene NFKBIA is associated with ectodermal dysplasia and other immune-related disorders when mutated, highlighting its critical role in immune homeostasis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser36, ensuring high specificity for the phosphorylated form. Non-phospho peptide blocking may be used to confirm phospho-specificity in WB.
• For WB, consider stimulating cells (e.g., with TNF-α or IL-1β) to induce IκBα phosphorylation and degradation, which can be monitored with this antibody.
• In IHC-P, antigen retrieval and permeabilization protocols should be optimized; validate staining in tissue sections known to express activated NF-κB signaling.
• For ELISA, use this antibody as a capture or detection reagent; cross-reactivity with unphosphorylated IκBα should be assessed depending on assay format.
• Because the antibody reacts with human, mouse, and rat, it is suitable for translational studies across these species; always include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated supply partner for IVD raw materials, offering ready-to-ship monoclonal antibodies, optimized antibody pairs, and ancillary reagents for NF-κB pathway research and diagnostic development. For this Phospho-IκBα-S36 antibody and related signaling targets, we can also source bulk quantities, rare specificities, and customized formats to streamline your assay pipeline from concept to clinic.