Phospho-HSP27/HSPB1-S82 Rabbit mAb for WB, ELISA - P04792

Core Product Specifications and Parameters

Biological Background: HSP27/HSPB1 Function and Localization

• Also known as 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, and Stress-responsive protein 27 (SRP27).
• Small heat shock protein that functions as a molecular chaperone, maintaining denatured proteins in a folding-competent state and playing a role in stress resistance and actin organization. Related references: PMID:10383393, PMID:20178975, PMID:19166925
• Through its chaperone activity, may regulate phosphorylation and axonal transport of neurofilament proteins. Related references: PMID:23728742
• Localizes to the cytoplasm, nucleus, and cytoskeleton, including spindle structures.
• Detected in all tissues, with highest expression in heart and striated/smooth muscle.
• Phosphorylation at Ser82 (and other residues) modulates HSPB1 chaperone function and client protein interactions.
• Mutations in HSPB1 are linked to Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide; always include phosphatase inhibitors in sample preparation to preserve phosphorylation state.
• For WB validation, treat lysates with lambda phosphatase—loss of signal confirms phospho-specificity.
• Optimize antibody dilution for your specific sample system; consider a titration series to determine the ideal working concentration.
• For ELISA, a phosphorylated peptide coating antigen is recommended to ensure phospho-specific detection.

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• CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials and technical services.
• This phospho-specific HSP27/HSPB1 antibody supports research in stress response, chaperone biology, and neurodegeneration.
• Our comprehensive catalog features validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your path from concept to clinic.