Phospho-HSP27/HSPB1-S15 Rabbit mAb - P04792

Core Product Specifications and Parameters

Biological Background: HSP27/HSPB1 Function and Localization

• HSP27, also known as heat shock protein beta-1, 28 kDa heat shock protein, estrogen-regulated 24 kDa protein, or stress-responsive protein 27 (SRP27), is encoded by the HSPB1 gene.
• It functions as a molecular chaperone that maintains denatured proteins in a folding-competent state, playing a crucial role in cellular stress responses. Related references: PMID:10383393, PMID:20178975
• HSP27 is involved in stress resistance and actin organization, contributing to cytoskeletal integrity under adverse conditions. Related references: PMID:19166925
• Through its chaperone activity, HSP27 may regulate diverse processes such as phosphorylation and axonal transport of neurofilament proteins. Related references: PMID:23728742
• Subcellularly, HSP27 localizes to the cytoplasm, nucleus, and cytoskeleton (specifically the spindle apparatus), enabling it to respond to and mitigate stress in multiple compartments.
• It is broadly expressed across tissues, with highest levels found in heart, skeletal muscle, and smooth muscle-rich organs; detected in serum and cerebrospinal fluid.
• Post‑translational modifications include phosphorylation, acetylation, and methylation, which regulate its chaperone activity, oligomeric state, and intracellular trafficking.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide spanning serine 15 of human HSP27. Pre‑absorption with non‑phosphorylated peptide is recommended to confirm phospho‑specificity.
• For Western blot, a band at approximately 23 kDa is expected; consider using a total HSP27 antibody as a loading control and to assess phosphorylation stoichiometry.
• In immunofluorescence, cellular stress (e.g., heat shock) may induce HSP27 phosphorylation and translocation to the nucleus or cytoskeleton—optimize fixation and permeabilization accordingly.
• Validate the antibody in the relevant sample system and application; titration may be necessary depending on endogenous expression levels and stress conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a dedicated IVD raw‑material sourcing partner, offering diagnostic manufacturers and research teams streamlined access to validated antibodies, antigens, and ancillary reagents. For HSP27‑related projects, we can supply optimized monoclonal/polyclonal antibodies (including phospho‑specific clones), bulk antibody pairs, and custom rare‑target sourcing—simplifying your transition from concept to clinic. Our technical team is ready to assist with product selection and application guidance to support your stress‑response or neurodegeneration research.