Phospho-HSF1-S326 Rabbit pAb for WB, ELISA - Q00613

Core Product Specifications and Parameters

Biological Background: HSF1 (Heat Shock Factor 1) Function and Localization

• HSF1 functions as a stress-inducible transcription factor that drives the expression of heat shock proteins (HSPs) to protect cells from damage. In unstressed cells, it is maintained as an inactive monomer in an HSP90 complex; upon stress, it trimerizes, binds heat shock elements (HSEs), and activates HSP gene transcription. Related references: PMID:11447121, PMID:12659875, PMID:12917326, PMID:11583998, PMID:16278218, PMID:9727490, PMID:10359787
• Upon heat shock stress, HSF1 forms a chromatin-associated complex with TTC5/STRAP and p300/EP300 to stimulate HSR transcription, thereby increasing cell survival. Related references: PMID:18451878
• HSF1 activates transcription of FOXR1, which in turn upregulates heat shock chaperones HSPA1A and HSPA6 and the antioxidant reductase DHRS2. Related references: PMID:34723967
• Beyond transcriptional activation, HSF1 represses Ras-induced c-fos transcription, promotes pre-mRNA 3'-end processing of HSP70, and mediates nuclear export of HSP70 mRNA. Related references: PMID:9341107, PMID:14707147, PMID:17897941
• HSF1 plays a role in mitotic progression and acts as a negative regulator of non-homologous end joining (NHEJ) DNA repair. Related references: PMID:18794143, PMID:26359349
• HSF1 is involved in latent HIV-1 reactivation by binding to the viral LTR promoter and recruiting transcriptional elongation factors.
• HSF1 localizes to the nucleus, cytoplasm, and associates with spindle poles, centrosomes, and kinetochores. It is regulated by phosphorylation at multiple sites, including serine 326, which is critical for its activation.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide encompassing serine 326 of human HSF1 and specifically recognizes the phosphorylated form. Include phosphatase inhibitors in lysis buffers to protect the epitope.
• For WB, the expected molecular weight is ~57 kDa. Use heat‑shocked vs. untreated human cell lysates as positive/negative controls. Consider treating samples with lambda phosphatase to confirm phospho‑specificity.
• In ELISA, optimize coating antigen concentration and antibody dilution. Pair with an appropriate detection system for quantitative analysis.
• Validate cross‑reactivity in your experimental system (human samples expected).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a one‑stop sourcing bridge for IVD raw materials, from single antibodies to bulk ancillary reagents and technical services. This phospho‑HSF1‑S326 antibody addresses heat shock and stress signaling research needs, and CamelBio can also assist in sourcing rare‑target raw materials or complementary reagents to accelerate your assay development.