Phospho-Histone H4-S1 Rabbit pAb for WB, ELISA - P62805

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Histone H4 is a core component of the nucleosome, wrapping and compacting DNA into chromatin.
• It plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
• H4 belongs to the histone family and is encoded by multiple genes, including H4C1 through H4C16.
• Post-translational modifications of H4, such as acetylation, methylation, phosphorylation, citrullination, and hydroxylation, form a "histone code" that regulates DNA accessibility.
• H4 is localized in the nucleus and on chromosomes.
• It has a molecular weight of 11367 Da (approximately 11 kDa) based on sequence.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around serine 1 of human Histone H4; antibody specificity for the phosphorylated form should be confirmed using phosphatase-treated controls in relevant sample systems.
• For Western blotting, consider appropriate loading controls and validate detection in the expected ~11 kDa range.
• In ELISA, include both phosphorylated and non-phosphorylated peptide controls to assess phospho-specificity.
• Cross-reactivity predicted with human, mouse, rat, and other species; independent validation in each species is recommended.

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CamelBio provides a comprehensive selection of IVD raw materials, including phospho-specific antibodies, validated antibody pairs, and bulk ancillary reagents for chromatin and histone modification research. With one-stop access to optimized monoclonals, polyclonals, and rare target sourcing, CamelBio supports diagnostic manufacturers and laboratories from concept to clinic. Explore how our supply capabilities can streamline your next development pipeline.