Phospho-Histone H3-T32 Rabbit pAb for WB, IHC-P, ELISA - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Histone H3.1t Function and Localization

• Histone H3.1t, also known as H3/g or Histone H3.4, is a variant of histone H3 encoded by the H3-4 gene (also designated H3FT, HIST3H3). It belongs to the histone H3 family, which includes several canonical and variant histones.
• H3.1t is a core component of nucleosomes, wrapping and compacting DNA into chromatin, thereby regulating transcription, DNA repair, DNA replication, and chromosomal stability.
• Histone H3 undergoes extensive post-translational modifications, including acetylation, methylation, phosphorylation, ADP-ribosylation, citrullination, hydroxylation, and ubiquitination, collectively known as the histone code, which modulates chromatin accessibility.
• H3.1t localizes to the nucleus and is associated with chromosomes.
• Tissue specificity: H3.1t is specifically expressed in testicular cells, suggesting a role in spermatogenesis.
• The H3.1t protein has a molecular weight of approximately 15.5 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr32; therefore, the antibody is expected to specifically recognize the phosphorylated form. Use phosphatase inhibitors during sample preparation to preserve the phosphorylation signal.
• For western blotting, validate the antibody in your cell line or tissue of interest, especially if the target is in low abundance or restricted to testis.
• In IHC-P, antigen retrieval conditions may need optimization; consider testing different retrieval methods.
• Cross-reactivity with mouse and rat indicates high sequence homology in the epitope region; however, confirm specificity with appropriate controls, as the antibody may detect other histone H3 variants if the epitope is conserved.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This Phospho-Histone H3-T32 Rabbit pAb is a high-quality tool for epigenetic and chromatin research. We offer validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development needs.