Phospho-Histone H3-T11 Rabbit pAb - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Histone H3.1t Function and Localization

• Histone H3.1t (H3/g, Histone H3.4) is a variant of the histone H3 family encoded by the H3-4 (H3FT, HIST3H3) gene in humans.
• As a core histone, H3.1t is a fundamental component of the nucleosome, the basic unit of chromatin, where DNA wraps around histone octamers to compact the genome.
• Nucleosomes regulate DNA accessibility, thereby playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
• Post-translational modifications of histones, including phosphorylation at specific residues such as threonine 11 (Thr11), constitute the "histone code" that modulates chromatin structure and function.
• H3.1t is localized to the nucleus and associates with chromosomes, consistent with its role in chromatin architecture.
• This variant is specifically expressed in testicular cells, suggesting a role in spermatogenesis or chromatin organization in the testis.
• The protein is subject to diverse modifications including acetylation, methylation, phosphorylation, and ubiquitination, as identified by proteomics and structural studies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr11 of human HIST1H3B, designed to generate phosphorylation-specific antibodies. Verify specificity with phosphatase-treated controls or peptide blocking.
• Recommended applications include WB, IHC-P, IF/ICC, and ELISA. For WB, the predicted molecular weight is ~15 kDa; however, histone modifications may alter migration. Use nuclear extracts or acid-extracted histones for enrichment.
• In IHC-P and IF/ICC, include appropriate controls (e.g., non-phosphopeptide blocking or phosphatase treatment) to confirm phosphorylation-dependent staining.
• Cross-reactivity is reported for human, mouse, and rat with wide predicted reactivity. Validate in your specific model system, as epitope conservation may vary.
• Store at -20°C upon receipt, and minimize freeze-thaw cycles to maintain antibody integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-phospho-Histone H3-T11 antibody supports research in epigenetics, cancer biology, and reproductive biology. Beyond this specific reagent, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD development workflows.