Phospho-Histone H3 (Ser28) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Histone H3.1t Function and Localization

• Core component of the nucleosome, wrapping and compacting DNA into chromatin, thereby limiting DNA accessibility and regulating transcription, DNA repair, DNA replication, and chromosomal stability.
• Post-translational modifications of histones, including phosphorylation at Ser28, constitute a "histone code" that dynamically controls chromatin structure and gene expression.
• Histone H3.1t (also known as H3/g or H3.4) is a testis-specific H3 variant encoded by the H3-4 gene, belonging to the histone H3 family.
• Localizes to the nucleus and associates with chromosomes, facilitating the organization of genomic DNA into nucleosomes.
• Subject to diverse post-translational modifications such as acetylation, methylation, phosphorylation, citrullination, ADP-ribosylation, hydroxylation, and ubiquitination, which collectively regulate chromatin function.
• Expressed primarily in testicular cells, indicating a specialized role in chromatin remodeling during spermatogenesis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues surrounding Ser28 of human Histone H3; verify that the antibody recognizes the phosphorylated form of the target and not the unmodified protein by using appropriate controls.
• For Western blotting (WB), consider analyzing mitotic cell lysates or samples treated with histone deacetylase inhibitors to enrich for phospho-H3 signal.
• In immunofluorescence (IF/ICC), phospho-H3 (Ser28) typically exhibits nuclear staining with chromosome-associated patterns during mitosis; co-stain with a DNA dye to confirm localization.
• When performing ELISA, immobilize the phosphopeptide antigen and compare binding to the non-phosphorylated counterpart to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to high-quality IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. This Phospho-Histone H3 (Ser28) antibody supports advanced chromatin and epigenetics research, and CamelBio offers bulk procurement and rare target sourcing to streamline your assay development from concept to clinic.