Phospho-Histone H3-S10 Rabbit mAb for WB, IHC-P, IP, DB, ELISA - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Histone H3.1t (H3/g, H3.4) Function and Localization

• Histone H3.1t is a core component of the nucleosome, wrapping and compacting DNA into chromatin to regulate DNA accessibility.
• It plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
• DNA accessibility is modulated through a complex set of post-translational modifications, collectively referred to as the histone code, along with nucleosome remodeling.
• Subcellular localization is predominantly nuclear and associated with chromosomes.
• This specific H3 variant is expressed in testicular cells, as indicated by tissue specificity data.
• The protein is subject to multiple post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination, as highlighted by keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser10 of human Histone H3; ensure specific recognition of the phosphorylated form by using appropriate phosphatase-treated controls or competitive peptides in your assay.
• For Western blotting (WB), standard protocols can be applied; consider using phospho-specific blockers and avoid phosphatase inhibitors in lysis buffers to preserve the phosphorylation status.
• In immunohistochemistry (IHC-P), antigen retrieval methods may be necessary; validate staining in your tissue of interest, given the broad predicted cross-reactivity.
• For immunoprecipitation (IP) and DNA-binding (DB) assays, optimize antibody concentration and incubation conditions to maintain phospho-epitope integrity.
• Cross-reactivity with human, mouse, and rat is expected; when working with other species, perform a preliminary validation due to "wide range predicted" cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. With this Phospho-Histone H3-S10 antibody, we facilitate advanced epigenetic and cell signaling research by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our expertise extends to rare target raw-material sourcing, ensuring you have reliable access to critical components for your assay development.