Phospho-Histone H2AX-S139 Rabbit pAb for WB, IF/ICC, IHC-P, ELISA - P16104

Core Product Specifications and Parameters

Biological Background: Histone H2AX Function and Localization

• Histone H2AX is a variant histone that replaces conventional H2A in a subset of nucleosomes, contributing to chromatin structure and DNA compaction.
• Nucleosomes containing H2AX regulate DNA accessibility, playing a central role in transcription, DNA repair, replication, and chromosomal stability.
• H2AX is critical for checkpoint-mediated cell cycle arrest in response to low-dose ionizing radiation and for efficient repair of DNA double-strand breaks (DSBs) through C-terminal phosphorylation at Ser139.
• Phosphorylation at Ser139 (γ-H2AX) serves as a key marker of DNA damage and facilitates the recruitment of repair proteins to DSB sites.
• The protein localizes to the nucleus and is associated with chromosomes.
• Post-translational modifications of H2AX include acetylation, phosphorylation, and ubiquitination, which modulate its function in DNA damage response and chromatin dynamics.
• Additional functional roles involve DNA recombination, meiosis, and host-virus interactions, as highlighted by UniProt keywords.

Experimental Guidance and Technical Tips

• For Western blot (WB) detection of phosphorylated H2AX, consider using phosphatase inhibitors in lysis buffers to preserve the S139 phosphorylation signal.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), optimize fixation and permeabilization conditions to achieve the best signal-to-noise ratio for nuclear targets.
• ELISA protocols may require careful validation of coating and detection antibody concentrations due to the small protein size (15 kDa).
• Validate the antibody in your specific model system, as cross-reactivity is reported for human, mouse, and rat, but performance may vary with sample type and processing.

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CamelBio offers diagnostic manufacturers and research laboratories a one-stop sourcing solution for IVD raw materials, including this anti-Phospho-Histone H2AX-S139 antibody for DNA damage and cell cycle research. Our supply capabilities encompass validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development and manufacturing needs.