Phospho-Histone H2AX-S139 Rabbit mAb for WB, IP, IF/ICC, ELISA - P16104

Core Product Specifications and Parameters

Biological Background: Histone H2AX Function and Localization

• Histone H2AX (H2A.X) is a variant histone H2A that replaces conventional H2A in a subset of nucleosomes.
• It plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability by compacting DNA into chromatin and regulating DNA accessibility through post-translational modifications.
• Phosphorylation at Ser139 (γ-H2AX) is critical for recognizing DNA double-strand breaks (DSBs) and recruiting DNA repair machinery, making it a key marker for DNA damage.
• Required for checkpoint-mediated cell cycle arrest in response to low doses of ionizing radiation and for efficient DSB repair.
• Localized in the nucleus and associated with chromosomes.
• Subject to multiple post-translational modifications including acetylation, phosphorylation, and ubiquitination, which fine-tune its function in DNA damage response.
• The gene H2AX (H2AFX) is a member of the histone H2A gene family and is widely conserved.
• Its predicted molecular weight is approximately 15 kDa, consistent with its typical migration on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S139 of human Histone H2AX; for phospho-specific detection, consider including phosphatase inhibitors during sample preparation.
• To validate the antibody's specificity for the phosphorylated form, compare signals from untreated vs. phosphatase-treated lysates.
• For Western blot, a band around 15 kDa is expected; ensure proper molecular weight markers are used.
• In immunofluorescence, γ-H2AX foci are a classic marker of DNA double-strand breaks; consider inducing DNA damage with agents like etoposide or ionizing radiation to enhance the signal.
• For ELISA, use a phosphorylated peptide as the coating antigen and a non-phosphorylated counterpart as a negative control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes one-stop access to IVD raw materials tailored for DNA damage and epigenetics research. This Phospho-Histone H2AX-S139 rabbit monoclonal antibody is part of our portfolio that includes validated primary antibodies, matched detection reagents, and ancillary raw materials to streamline your assay development. From concept to clinic, CamelBio bridges the gap between target discovery and reliable supply for your most demanding projects.