Phospho-HER2/ErbB2-Y1139 Rabbit mAb for WB and ELISA - P04626

Core Product Specifications and Parameters

Biological Background: HER2/ErbB2 Function and Localization

• HER2 (Receptor tyrosine-protein kinase erbB-2) is a protein tyrosine kinase that forms part of cell surface receptor complexes, requiring a coreceptor for ligand binding. It is essential for neuregulin-receptor signaling, though neuregulins do not bind it directly.
• It regulates outgrowth and stabilization of peripheral microtubules via the MEMO1-RHOA-DIAPH1 pathway, leading to GSK3B inhibition, APC and CLASP2 membrane association, and microtubule capture by MACF1.
• In the nucleus, HER2 acts as a transcription factor, binding the PTGS2/COX-2 promoter at 5'-TCAAATTC-3' and activating its transcription, and is implicated in CDKN1A activation involving STAT3 and SRC.
• It also enhances rRNA transcription by RNA Pol I, promoting protein synthesis and cell growth.
• Subcellularly, HER2 localizes to the cell membrane, ruffles, early endosomes, cytoplasm (perinuclear), and nucleus, consistent with its dual signaling and transcriptional roles.
• Tissue specificity: Expressed in various tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney, and mouth.
• Key features: It is a single-pass type I membrane glycoprotein with kinase activity, containing an ATP-binding site, phosphorylation sites, and disulfide bonds. It is involved in chromosomal rearrangements, alternative splicing, and disease variants.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphorylated peptide surrounding tyrosine 1139 of human HER2. For Western blot, consider using phosphorylation-specific blocking conditions and appropriate phosphatase inhibitors during sample preparation to preserve the phospho-epitope.
• Given the predicted molecular weight of 138 kDa, ensure adequate gel separation and transfer conditions for high molecular weight proteins in Western blotting.
• For ELISA, it is advisable to validate the antibody with phosphorylated and non-phosphorylated peptides to confirm specificity. Include appropriate positive and negative controls, such as lysates from HER2-overexpressing and phosphatase-treated cell lines.
• Cross-reactivity is reported for human; verify reactivity with other species if needed. Since HER2 is a transmembrane receptor, consider using membrane-enriched fractions for enhanced detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for diagnostic assay development. This phospho-HER2 (Y1139) rabbit monoclonal antibody supports research into HER2 signaling pathways, particularly in oncology biomarker discovery. For your unique development needs, CamelBio can source rare-target raw materials and provide tailored procurement solutions.