Phospho-HDAC4-S632 Rabbit Polyclonal Antibody for WB - P56524

Core Product Specifications and Parameters

Biological Background: HDAC4 Function and Localization

• Histone deacetylase 4 (HDAC4) is a member of the class II histone deacetylase family, encoded by the HDAC4 gene. It functions as a transcriptional co-repressor by removing acetyl groups from the N-terminal lysine residues of core histones (H2A, H2B, H3, and H4), leading to chromatin condensation and epigenetic silencing.
• HDAC4 plays a pivotal role in numerous biological processes including transcriptional regulation, cell cycle progression, and developmental events. It often operates within large multiprotein complexes to exert its deacetylase activity.
• This protein is critically involved in muscle maturation by interacting with myocyte enhancer factors such as MEF2A, MEF2C, and MEF2D, thereby influencing muscle gene expression programs.
• HDAC4 participates in the MTA1-mediated epigenetic regulation of ESR1 (estrogen receptor alpha) expression in breast cancer, highlighting its relevance in oncogenic pathways.
• Beyond histones, HDAC4 deacetylates non-histone substrates such as HSPA1A and HSPA1B at Lys-77, which modulates their binding to the co-chaperone STUB1. Related references: PMID:27708256
• Subcellular localization is dynamic: HDAC4 shuttles between the nucleus and the cytoplasm in response to cellular signals, a process tightly regulated by phosphorylation.
• The protein is ubiquitously expressed across tissues, reflecting its broad functional significance.
• Annotated with keywords including phosphoprotein, alternative splicing, chromatin regulator, and disease variants, HDAC4 is linked to conditions such as epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• This antibody was generated using a phospho-specific peptide immunogen corresponding to residues surrounding serine 632 of human HDAC4. It is intended to recognize only the phosphorylated form of HDAC4 at S632.
• For Western blotting, consider treating cell lysates with phosphatase inhibitors to preserve the phosphorylation signal. A comparative blot using phosphatase-treated samples (e.g., λ-phosphatase) can help confirm phospho-specificity.
• The antibody is reported to cross-react with mouse and rat HDAC4. However, we recommend verifying cross-reactivity and optimal dilution in your specific model system through individual validation.
• Given the high molecular weight (119 kDa), use an appropriate gel percentage and transfer conditions to ensure efficient detection. Load adequate protein amounts and consider extended transfer times for complete membrane binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement experience for diagnostic manufacturers, laboratories, and research institutes seeking high-quality IVD raw materials. This Phospho-HDAC4-S632 antibody supports research in epigenetic regulation and chromatin biology, and we can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with sourcing challenging targets. Partner with CamelBio for reliable access to the critical materials that drive your diagnostic and research programs forward.