Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P56524 / Q9UQL6 / Q9UKV0

Core Product Specifications and Parameters

Biological Background: HDAC4/HDAC5/HDAC9 Function and Localization

• HDAC4 is a histone deacetylase that catalyzes the removal of acetyl groups from lysine residues on the N-terminal tails of core histones (H2A, H2B, H3, H4), leading to chromatin compaction and epigenetic repression.
• Histone deacetylation by HDAC4 plays a critical role in transcriptional regulation, cell cycle progression, and developmental processes through the formation of large multiprotein complexes.
• HDAC4 is involved in muscle maturation by interacting with myocyte enhancer factors MEF2A, MEF2C, and MEF2D, thereby modulating muscle gene expression.
• It participates in MTA1-mediated epigenetic regulation of estrogen receptor 1 (ESR1) expression in breast cancer.
• HDAC4 deacetylates HSPA1A and HSPA1B at Lys-77, promoting their binding to the co-chaperone STUB1. Related references: PMID:27708256
• The protein is classified as a phosphoprotein; phosphorylation at specific serine residues (including S246 in HDAC4, S259 in HDAC5, S220 in HDAC9) regulates its subcellular localization and repressor activity. (UniProt keyword: Phosphoprotein)
• HDAC4 localizes to both the nucleus and cytoplasm, with shuttling between compartments modulated by post-translational modifications.
• It is ubiquitously expressed across human tissues, highlighting its broad regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen is a phospho-specific peptide surrounding S246 of human HDAC4; the antibody is expected to recognize phosphorylated HDAC4, HDAC5, and HDAC9 in human, mouse, and rat samples.
• For Western blot, expect major bands around 97–122 kDa (HDAC4/5) and potentially 57–65 kDa (HDAC9 isoform); use appropriate phospho-protein extraction and loading controls.
• In IHC-P, consider antigen retrieval and validate staining specificity with phosphatase treatment or phospho-blocking peptides.
• For ELISA, include both non-phosphorylated and phosphorylated peptide standards to confirm phosphorylation-dependent binding.
• As a rabbit polyclonal, lot-to-lot consistency should be verified; validate with positive and negative cell lysates (e.g., stimulated vs. unstimulated) in the relevant sample system.

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CamelBio is your one-stop IVD raw-material partner, supplying diagnostic manufacturers and research laboratories with high-quality reagents like this phospho-HDAC4/5/9 antibody for epigenetics and transcription studies. We offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your diagnostic assay development from concept to clinic.