Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb - P49840 / P49841

Core Product Specifications and Parameters

Biological Background: GSK3α/GSK3β Function and Localization

• GSK3A encodes glycogen synthase kinase-3 alpha, a constitutively active serine/threonine kinase that negatively regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), thereby controlling glucose homeostasis. Related references: PMID:11749387, PMID:17478001, PMID:19366350
• In the Wnt signaling pathway, GSK3α phosphorylates CTNNB1/beta-catenin, APC, and AXIN1, regulating beta-catenin stability and transcriptional activity. Related references: PMID:17229088
• GSK3α is implicated in Alzheimer's disease through facilitation of amyloid precursor protein (APP) processing and generation of amyloid-beta plaques. Related references: PMID:12761548
• It plays a role in insulin signaling and may contribute to insulin resistance by regulating transcription factor activation. Related references: PMID:10868943, PMID:17478001
• GSK3α regulates mTORC2 signaling by phosphorylating RICTOR at Thr-1695, promoting FBXW7-mediated ubiquitination and degradation of RICTOR. Related references: PMID:25897075
• Under starvation, GSK3α mediates phosphorylation of KAT5/TIP60, activating its acetyltransferase activity and promoting autophagy. Related references: PMID:30704899
• The closely related isoform GSK3β (GSK3B) shares similar substrate specificity and is often co‑regulated with GSK3α in many signaling pathways.
• Keywords include ATP-binding, kinase, phosphoprotein, Wnt signaling, neurogenesis, diabetes, and Alzheimer disease, reflecting its diverse roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide covering Y216 of GSK3β and Y279 of GSK3α; for phospho‑specific detection, include phosphatase inhibitors during sample preparation and validate with phosphatase treatment controls.
• For Western blot (WB), expect a band at ~47 kDa; optimize blocking and antibody dilution to reduce non‑specific binding.
• In immunohistochemistry (IHC‑P), ensure proper antigen retrieval and use phospho‑specific controls to confirm signal specificity.
• The antibody cross‑reacts with human, mouse, and rat; therefore, signal may be detected across these species—validate in your model system.
• ELISA can be used for quantitative detection; paired with a total protein antibody, it can assess phosphorylation status.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one‑stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. As a B2B provider specializing in phospho‑signaling and metabolic disease targets like GSK3α/β, we streamline procurement from concept to clinic. Contact us for rare target sourcing and technical consulting tailored to your diagnostic development needs.