Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit Monoclonal Antibody for WB, ELISA - P49840/P49841

Core Product Specifications and Parameters

Biological Background: GSK3α Function and Localization

• Glycogen synthase kinase-3 alpha (GSK3A) is a constitutively active serine/threonine-protein kinase encoded by the GSK3A gene; it belongs to the GSK3 family, which also includes the closely related beta isoform (GSK3B).
• GSK3A acts as a key negative regulator in insulin-mediated glucose homeostasis by phosphorylating and inhibiting glycogen synthase (GYS1), thereby suppressing glycogen synthesis. Related references: PMID:11749387, PMID:17478001, PMID:19366350
• In the canonical Wnt signaling pathway, GSK3A phosphorylates beta-catenin (CTNNB1), APC, and AXIN1, leading to beta-catenin degradation and reduced transcriptional activity. Related references: PMID:17229088
• GSK3A contributes to Alzheimer’s disease pathology by facilitating amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques. Related references: PMID:12761548
• Under starvation conditions, GSK3A promotes autophagy through phosphorylation of KAT5/TIP60, enhancing its acetyltransferase activity and subsequent acetylation of ULK1 and RUBCNL. Related references: PMID:30704899
• GSK3A phosphorylates mTORC2 component RICTOR at Thr-1695, triggering FBXW7-mediated ubiquitination and degradation, thus modulating mTORC2 signaling. Related references: PMID:25897075
• Additional roles include regulation of neuronal polarity, axon outgrowth, and apoptosis; GSK3A is also implicated in the development of insulin resistance and pancreatic beta-cell replication.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phospho-peptide encompassing the Y216 phosphorylation site of GSK3β and Y279 of GSK3α; it is expected to specifically detect phosphorylated forms at these residues. For Western blot, include phosphatase inhibitors during sample preparation to preserve phosphorylation states.
• The antibody demonstrates reactivity with both human and mouse proteins, suggesting conservation of the epitope across species. Validate antibody specificity in your target tissue or cell line using positive and negative controls (e.g., phosphatase treatment).
• For ELISA applications, consider using phosphorylated peptide as coating antigen and non-phosphorylated peptide to assess phospho-specificity.
• Given its rabbit monoclonal origin, expect low background and high batch-to-batch consistency. Optimize dilution conditions empirically for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete sourcing solution for IVD manufacturers and researchers, offering validated antibody pairs, high-quality monoclonal and polyclonal antibodies, and bulk ancillary reagents. This phospho-GSK3α/β (Y279/Y216) antibody supports critical studies in metabolic signaling, neurodegeneration, and cancer. Reach out to explore custom sourcing for rare‑target raw materials and let us accelerate your development from concept to clinic.