Phospho-GSK3β-S9 Rabbit pAb - P49841

Core Product Specifications and Parameters

Biological Background: GSK3β Function and Localization

• GSK3β (Glycogen synthase kinase-3 beta) is a constitutively active serine/threonine-protein kinase that negatively regulates glycogen synthesis, Wnt signaling, and transcription factors by phosphorylating targets such as glycogen synthase, beta-catenin, and JUN. Related references: PMID:11430833, PMID:12554650, PMID:14690523, PMID:1846781
• In the Wnt pathway, GSK3β forms a destruction complex with APC and AXIN1, phosphorylating beta-catenin to target it for ubiquitin-mediated proteasomal degradation. Related references: PMID:12554650
• Phosphorylation of microtubule-associated protein TAU at Thr-548 by GSK3β reduces TAU’s ability to stabilize microtubules, a process linked to neurofibrillary tangle formation in Alzheimer’s disease. Related references: PMID:14690523
• GSK3β localizes to the cytoplasm, nucleus, and cell membrane, enabling its involvement in multiple signaling cascades.
• It is expressed in testis, thymus, prostate, and ovary, with lower expression in lung, brain, and kidney; in Alzheimer’s disease brains, it colocalizes with EIF2AK2/PKR and TAU.
• The kinase also regulates circadian rhythm by phosphorylating clock components BMAL1 and CLOCK, and modulates autophagy via KAT5/TIP60 acetylation under starvation. Related references: PMID:19946213, PMID:28903391, PMID:30704899

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide corresponding to human GSK3β around Ser9; confirm phospho-specificity by treating samples with lambda phosphatase or using a non-phospho peptide competition assay.
• The predicted molecular weight of GSK3β is approximately 47 kDa; optimize SDS-PAGE gel percentage and transfer conditions for Western blot to achieve clear band separation.
• For immunohistochemistry (IHC-P), consider heat-induced antigen retrieval in citrate buffer (pH 6.0) and titrate the primary antibody to maximize signal-to-noise ratio.
• In immunofluorescence (IF/ICC), validate fixation and permeabilization protocols for your cell system, as GSK3β can localize to multiple subcellular compartments.
• Cross-reactivity with mouse and rat has been reported; verify reactivity in your target species tissue or cell lysate before large-scale experiments.

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CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated phospho-specific antibodies, antibody pairs, and bulk ancillary reagents. Whether you are developing assays for Wnt signaling, Alzheimer’s disease, or metabolic disorder biomarkers, CamelBio supports your entire workflow—from concept to clinic—with expert technical sourcing and consultation. For rare target raw materials, trust CamelBio to connect you with reliable supply solutions.