Phospho-GSK3α-S21+GSK3β-S9 Rabbit mAb for WB, ELISA - P49840/P49841

Core Product Specifications and Parameters

Biological Background: GSK3α/GSK3β Function and Localization

• Glycogen synthase kinase-3 alpha (GSK3A) is a constitutively active serine/threonine-protein kinase that requires primed phosphorylation of its substrates, serving as a key negative regulator in insulin-mediated glycogen synthesis by phosphorylating and inhibiting glycogen synthase (GYS1) (PubMed:11749387, PubMed:17478001, PubMed:19366350). Related references: PMID:11749387 PMID:17478001 PMID:19366350
• In the Wnt signaling pathway, GSK3A phosphorylates CTNNB1/beta-catenin, APC, and AXIN1, thereby controlling beta-catenin stability and transcriptional activity (PubMed:17229088). Related references: PMID:17229088
• GSK3A contributes to Alzheimer disease pathology by facilitating APP processing and the generation of amyloid-beta peptides (PubMed:12761548). Related references: PMID:12761548
• The kinase modulates autophagy under starvation conditions by phosphorylating KAT5/TIP60, which enhances its acetyltransferase activity toward key autophagy regulators ULK1 and RUBCNL/Pacer (PubMed:30704899). Related references: PMID:30704899
• GSK3A phosphorylates RICTOR at Thr-1695, promoting FBXW7-mediated ubiquitination and degradation of this mTORC2 component, thus regulating mTOR signaling (PubMed:25897075). Related references: PMID:25897075
• It negatively regulates extrinsic apoptotic signaling by forming an anti-apoptotic complex with DDX3X and BRIC2 at death receptors, with this function most effective under weak apoptotic stimuli.
• According to UniProt, GSK3A is associated with keywords such as Acetylation, Phosphoprotein, Diabetes mellitus, Neurogenesis, and Alzheimer disease, indicating regulation by post-translational modifications and involvement in diverse cellular processes.

Experimental Guidance and Technical Tips

• This antibody was generated using a synthetic phosphorylated peptide encompassing Ser21 of GSK3α and Ser9 of GSK3β, suggesting specificity for the phosphorylated forms of both kinases. For Western blot, consider treating cell lysates with lambda phosphatase to confirm phospho-specificity.
• Based on the application data, the antibody is recommended for WB and ELISA. When performing WB, include appropriate positive and negative controls (e.g., insulin-stimulated vs. serum-starved cells) to verify phosphorylation status.
• The antibody cross-reacts with mouse, supporting its use in mouse models of metabolic disease, neurodegeneration, or cancer, where GSK3α/β signaling is studied.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this phospho-GSK3α/β monoclonal antibody as a high-quality raw material for diagnostic and research applications targeting glucose metabolism, Wnt signaling, and neurodegeneration pathways. Leveraging a comprehensive supply network, CamelBio offers one-stop access to validated antibody pairs, bulk ancillary reagents, and specialized technical consulting to accelerate your IVD assay development from concept to clinical application.