Phospho-GRIN2B-Y1474 Rabbit Polyclonal Antibody for WB, ELISA - Q13224

Core Product Specifications and Parameters

Biological Background: GRIN2B Function and Localization

• GRIN2B encodes the GluN2B (NR2B) subunit of N-methyl-D-aspartate (NMDA) receptors, which are heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg2+. Related references: PMID:24272827, PMID:24863970, PMID:26875626
• Channel activation requires simultaneous binding of glutamate to GluN2, glycine or D-serine to GluN1, and membrane depolarization to relieve Mg2+ block, allowing Ca2+/Na+ influx and K+ efflux. Related references: PMID:24272827, PMID:24863970, PMID:26875626
• The GluN2B subunit confers distinct channel properties, including activation/deactivation kinetics, pH sensitivity, Ca2+ permeability, and interactions with allosteric modulators. Related references: PMID:26875626, PMID:28095420, PMID:28126851
• Predominantly expressed in the fronto-parieto-temporal cortex and hippocampal pyramidal cells, with lower levels in the basal ganglia, supporting roles in learning, memory, and cognition.
• Subcellular localization includes the postsynaptic cell membrane, dendritic projections, late endosomes, lysosomes, and cytoskeleton, reflecting dynamic trafficking and regulated degradation.
• Phosphorylation at Ser-1303 by DAPK1 at extrasynaptic sites enhances NMDA receptor activity, contributing to excitotoxic Ca2+ overload and irreversible neuronal death in stroke models.
• Mutations in GRIN2B are associated with developmental and epileptic encephalopathy (DEE27, EIEE27) and intellectual disability (MRD6), underscoring its critical neurological roles.

Experimental Guidance and Technical Tips

• This phospho-specific antibody targets Y1474 of human GRIN2B; confirm specificity using non-phosphorylated peptide or phosphatase-treated lysates as negative controls.
• For WB, the predicted molecular weight is 166 kDa; use adequate transfer conditions and consider enrichment for membrane proteins if signal is low.
• Include phosphatase inhibitors during sample preparation to preserve phosphorylation status.
• Validate antibody performance in physiologically relevant human cell lines or brain tissues known to express GRIN2B (e.g., SH-SY5Y, hippocampal lysates).
• ELISA applications may benefit from using a phospho-peptide as coating antigen and comparing reactivity with the unphosphorylated form.

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CamelBio provides diagnostic manufacturers and research laboratories with consolidated access to premium IVD raw materials, including validated phospho-specific antibodies like this anti-phospho-GRIN2B (Y1474) antibody, to streamline neuroscience assay development. Beyond targeted reagents, we supply optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets such as ion channels and synaptic proteins. Partner with CamelBio to advance your projects from concept to clinic with reliable, one-stop raw-material solutions.